	   SnapGene      $3CGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCGCGCAATTAACCCTCACTAAAGGGAACAAAAGCTGGAGCTGCAAGCTTAATGTAGTCTTATGCAATACTCTTGTAGTCTTGCAACATGGTAACGATGAGTTAGCAACATGCCTTACAAGGAGAGAAAAAGCACCGTGCATGCCGATTGGTGGAAGTAAGGTGGTACGATCGTGCCTTATTAGGAAGGCAACAGACGGGTCTGACATGGATTGGACGAACCACTGAATTGCCGCATTGCAGAGATATTGTATTTAAGTGCCTAGCTCGATACATAAACGGGTCTCTCTGGTTAGACCAGATCTGAGCCTGGGAGCTCTCTGGCTAACTAGGGAACCCACTGCTTAAGCCTCAATAAAGCTTGCCTTGAGTGCTTCAAGTAGTGTGTGCCCGTCTGTTGTGTGACTCTGGTAACTAGAGATCCCTCAGACCCTTTTAGTCAGTGTGGAAAATCTCTAGCAGTGGCGCCCGAACAGGGACTTGAAAGCGAAAGGGAAACCAGAGGAGCTCTCTCGACGCAGGACTCGGCTTGCTGAAGCGCGCACGGCAAGAGGCGAGGGGCGGCGACTGGTGAGTACGCCAAAAATTTTGACTAGCGGAGGCTAGAAGGAGAGAGATGGGTGCGAGAGCGTCAGTATTAAGCGGGGGAGAATTAGATCGCGATGGGAAAAAATTCGGTTAAGGCCAGGGGGAAAGAAAAAATATAAATTAAAACATATAGTATGGGCAAGCAGGGAGCTAGAACGATTCGCAGTTAATCCTGGCCTGTTAGAAACATCAGAAGGCTGTAGACAAATACTGGGACAGCTACAACCATCCCTTCAGACAGGATCAGAAGAACTTAGATCATTATATAATACAGTAGCAACCCTCTATTGTGTGCATCAAAGGATAGAGATAAAAGACACCAAGGAAGCTTTAGACAAGATAGAGGAAGAGCAAAACAAAAGTAAGACCACCGCACAGCAAGCGGCCGCTGATCTTCAGACCTGGAGGAGGAGATATGAGGGACAATTGGAGAAGTGAATTATATAAATATAAAGTAGTAAAAATTGAACCATTAGGAGTAGCACCCACCAAGGCAAAGAGAAGAGTGGTGCAGAGAGAAAAAAGAGCAGTGGGAATAGGAGCTTTGTTCCTTGGGTTCTTGGGAGCAGCAGGAAGCACTATGGGCGCAGCGTCAATGACGCTGACGGTACAGGCCAGACAATTATTGTCTGGTATAGTGCAGCAGCAGAACAATTTGCTGAGGGCTATTGAGGCGCAACAGCATCTGTTGCAACTCACAGTCTGGGGCATCAAGCAGCTCCAGGCAAGAATCCTGGCTGTGGAAAGATACCTAAAGGATCAACAGCTCCTGGGGATTTGGGGTTGCTCTGGAAAACTCATTTGCACCACTGCTGTGCCTTGGAATGCTAGTTGGAGTAATAAATCTCTGGAACAGATTTGGAATCACACGACCTGGATGGAGTGGGACAGAGAAATTAACAATTACACAAGCTTAATACACTCCTTAATTGAAGAATCGCAAAACCAGCAAGAAAAGAATGAACAAGAATTATTGGAATTAGATAAATGGGCAAGTTTGTGGAATTGGTTTAACATAACAAATTGGCTGTGGTATATAAAATTATTCATAATGATAGTAGGAGGCTTGGTAGGTTTAAGAATAGTTTTTGCTGTACTTTCTATAGTGAATAGAGTTAGGCAGGGATATTCACCATTATCGTTTCAGACCCACCTCCCAACCCCGAGGGGACCCGACAGGCCCGAAGGAATAGAAGAAGAAGGTGGAGAGAGAGACAGAGACAGATCCATTCGATTAGTGAACGGATCTCGACGGTATCGGTTAACTTTTAAAAGAAAAGGGGGGATTGGGGGGTACAGTGCAGGGGAAAGAATAGTAGACATAATAGCAACAGACATACAAACTAAAGAATTACAAAAACAAATTACAAAATTCAAAATTTTATCGATAAGCTTGGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAGATCGCCTGGAGACGCCATCCACGCTGTTTTGACCTCCATAGAAGACACCGACTCTAGAGCCACCATGGCCAGCAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCTACATACGGAAAGCTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCTCTTATGGTGTTCAATGCTTTTCCCGTTATCCGGATCATATGAAACGGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAACGCACTATATCTTTCAAAGATGACGGGAACTACAAGACGCGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATCGTATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTCGGACACAAACTCGAGTACAACTATAACTCACACAATGTATACATCACGGCAGACAAACAAAAGAATGGAATCAAAGCTAACTTCAAAATTCGCCACAACATTGAAGATGGATCCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCGACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGCGTGACCACATGGTCCTTCTTGAGTTTGTAACTGCTGCTGGGATTACACATGGCATGGATGAGCTCTACAAAAGCGGTTCCggaCCGGATCAAACAAGTTTGTACAAAAAAGCAGGCTTACTACAGCTACCGCCGCTGGAAAGACTGACTCTGGGAGGCGGGATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAGGGATCCGGACTAGTTAACGCGTCGACCAGTGGTCGACCCTGTGGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCGGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAAAGCTTACCATGACCGAGTACAAGCCCACGGTGCGCCTCGCCACCCGCGACGACGTCCCCAGGGCCGTACGCACCCTCGCCGCCGCGTTCGCCGACTACCCCGCCACGCGCCACACCGTCGATCCGGACCGCCACATCGAGCGGGTCACCGAGCTGCAAGAACTCTTCCTCACGCGCGTCGGGCTCGACATCGGCAAGGTGTGGGTCGCGGACGACGGCGCCGCGGTGGCGGTCTGGACCACGCCGGAGAGCGTCGAAGCGGGGGCGGTGTTCGCCGAGATCGGCCCGCGCATGGCCGAGTTGAGCGGTTCCCGGCTGGCCGCGCAGCAACAGATGGAAGGCCTCCTGGCGCCGCACCGGCCCAAGGAGCCCGCGTGGTTCCTGGCCACCGTCGGCGTCTCGCCCGACCACCAGGGCAAGGGTCTGGGCAGCGCCGTCGTGCTCCCCGGAGTGGAGGCGGCCGAGCGCGCCGGGGTGCCCGCCTTCCTGGAGACCTCCGCGCCCCGCAACCTCCCCTTCTACGAGCGGCTCGGCTTCACCGTCACCGCCGACGTCGAGGTGCCCGAAGGACCGCGCACCTGGTGCATGACCCGCAAGCCCGGTGCCTGACGCCCGCCCCACGACCCGCAGCGCCCGACCGAAAGGAGCGCACGACCCCATGCATCGCCAGTCGACAATCAACCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTTGCTCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTTCCCGTATGGCTTTCATTTTCTCCTCCTTGTATAAATCCTGGTTGCTGTCTCTTTATGAGGAGTTGTGGCCCGTTGTCAGGCAACGTGGCGTGGTGTGCACTGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCATTGCCACCACCTGTCAGCTCCTTTCCGGGACTTTCGCTTTCCCCCTCCCTATTGCCACGGCGGAACTCATCGCCGCCTGCCTTGCCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGTCGGGGAAGCTGACGTCCTTTCCATGGCTGCTCGCCTGTGTTGCCACCTGGATTCTGCGCGGGACGTCCTTCTGCTACGTCCCTTCGGCCCTCAATCCAGCGGACCTTCCTTCCCGCGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCGTCTTCGCCTTCGCCCTCAGACGAGTCGGATCTCCCTTTGGGCCGCCTCCCCGCCTGGAATTCGAGCTCGGTACCTTTAAGACCAATGACTTACAAGGCAGCTGTAGATCTTAGCCACTTTTTAAAAGAAAAGGGGGGACTGGAAGGGCTAATTCACTCCCAACGAAGACAAGATCTGCTTTTTGCTTGTACTGGGTCTCTCTGGTTAGACCAGATCTGAGCCTGGGAGCTCTCTGGCTAACTAGGGAACCCACTGCTTAAGCCTCAATAAAGCTTGCCTTGAGTGCTTCAAGTAGTGTGTGCCCGTCTGTTGTGTGACTCTGGTAACTAGAGATCCCTCAGACCCTTTTAGTCAGTGTGGAAAATCTCTAGCAGTAGTAGTTCATGTCATCTTATTATTCAGTATTTATAACTTGCAAAGAAATGAATATCAGAGAGTGAGAGGAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGGCTCTAGCTATCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCGGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGGACGTACCCAATTCGCCCTATAGTGAGTCGTATTACGCGCGCTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTTAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAG  Ng   +   1          9   5   $   "   *             '   /   '   /   )   (   &   9   4   ,      $            (   *   "      0   ,   (      "         !                              )         /   7   6      "   :      +   #   *   (   0   A   &   )      )   *   3   '   %   '   %   $      3   2   6      ,   :      0   :   I   ,      $   "   0      "   -   3      1                %         $   +   '                  "         !   "   ?   !      !      &   "   $   1      )   *   ?   +   2   ,   !      .   &               &   /   (         $         %      &         *         ,         "   "      (      ,   %   $   )   .   R   (      &       .            %      -         (                   !   *            1   !         &               %   %      Y   !   -       0   3   4   &   6   &         #         $   .   G   )       $               #      :   *         '          &      F   '   !   $   0   1      4       4   .   2   ,        + stŸ ü?GÊ;<=DEFS
LË{|NvŸµ¶·~»ùú @ w"x"y"##n#o#p#1 ';ËÜuˆ¡ÍßOm@°žË>T¯	´	
@
\
ž
?³$+Úý. Ù¸%dâO¼ûA Ø"## ¶ZeHG3	iM9AOòŒ¾ ¬ùB"z"ú">#  [ Ä‚Òý
Š
O9�–‰ªÌ´+} w„XÂ€Ñ+ n"q#9 i(çEPvÎÏÒÕèVn}«`ð°7	A
Ÿ
Î
%d%?J¹×ÇVblä6;_úÇúÓÞ¡êB ’"Ù"##u#5  T ¶ Ô 	‰¨<®Ý‰¢”Pw!Rq�˜%ÞU #@™ç(?‡ÆQ¡i¾&˜ìe"½É'ÿ" #~#-$$ Â Á,i0A§Ÿ?


ê´Äâ,Ûþ%eÎLP»~ü�§R Ì .!„!\"Ü#" ÆÌøà±Ì	µ	]
¥Ýf_¼4ëôHb®/qÚ¹ãÖ´G"ã"* †‘)[7í4	è	›
CjpÐc0NQ}•:‚”<W�P¤»Ïè–
¿®‚¡ðq Ó u! J”‰š³Ch!HÈB	‰	Ž
„AvsÈ  ‡ ¢!è"ï"*#  H ”fI+l'ð	
×©ï´
üK�Ååh­ZÖx!C"{"?#œ# ·	¢
—]¾B]ó9:új"û"' ¿"ƒÜ
‹
’
>(:j‹‘—÷ŠeOZ~Ò >ãNo Ûór¤-"b"à"r#/ ? \ ˆ ¬Å(þšR	™	Ã	ö	M
PøÔ¿¦Çf«ÀÍ8æWŸ7!Û…‘¢±[,‚V‹I ö"' ‘ ¦ 2þHÿÓ=cØþ?K“Yk,°…Š”x}¶Ãp¼e�ÎÒ©! "o"/ * šê» ÖÏ�		³DvÚûk+1:eµ‘œ´ÊÝöAÏó%YÙßèJ°Ò`ºÅ ß!‘#£#®#) åF’ÐÓÝÐÚaÈ 
g&=Ž”{‡º2)/TWc<¢Åê×Ý®Ô¢#+$( jlâ‘QSU`wnÖé.=?¶ñóõ÷ýÏ
e­m‹`{âèþÔ�k 8!ª"Á"Ú"#& v S`,3~¬”±8	B
&{±¡ØÈ† 7ÈGJä3û+1¨Ö^«C !ò#$9  b ¾ !)èbÏ˜HWo°
ÿ
È3@aK¢‹�ïAo¥åE�ûpïs÷1ß9ëó!‹!³!“"º"##S#v#ö#$'$4  Š©Mü2€•ë0xßÛäçêï
mT`“´<€§¯[£LvÇ«¢¿Õú«(•ãÛ'Ö Ÿ!Õ".$, U Õ n¯ÒØÞŠ£§óA"S¸ô6V€¡óšy†@ˆö5°™Õf¾!z…pZ "!Ã"Ü"!## · ÒyðQr™âù&s�ßZ€:ãè)jÚ×Þ�Q‘#$ 
Ê=ÆÕ5ÄŽý½
$A˜
XR'í#Ê¶æuV í!s"—"¥" #$ PW…¨ë	@Fs9–AÅ-šÃÂ�ôGž S c# Ã äýjpŽØ&Y ¦
ffÞ¯¨ám ã º!0"R"]"¬"Ý#û# . æ ‚ÂÙB“@ 
Šµ&9Ï“¹FQZx| ¬ ° Z#Ô#( ™“-<Â1Æ
ë}ã"¶Üÿï�M›Ý}º˜¼ÍÏýd Í Ù '!/!:!…!* ³Çò‘—õ	*	}	†	7
�
Ñ
<Jgm`c½5hì§ªILœÖû¨ÛDäâô%6= O"" D ¼(w‰á¥²Òùÿbqò
¦ØVg�ÿ}1r‹Ø ‡É?` ä" P ƒÍù%>	¶	
^
ø
ý$±Ô=0ˆºmÀ`U™H"N#0 à œ©Ê¢¶ÍÜç¬	å
#�Þ®çðqJ¤Ð ‚õcb¯Âäò�†ª×µ’ î !›"$, ´ ‡’¬½5M®#Aî5	
œ
bO&=…?Xí9cÍáæ”–IÏ¯ª÷v!"+"Ú#( ` t ¼ �*î�é	»
Dkqd�1å~–ÎR3kæ 1^Àƒ­=ñÔ ÿ ¸"a#ô#%$ ² npª]_	•
Ñ…-/;ƒ=‚éò—ÂXZi”BÐ!8"" y�òbr\_8¹jN
ORÓúÿ>q•-Q¥¼ÐëL\™Ã¢›r €" &;kM]i_�
úO˜U§ªýîÌN/ !Ò!Ø!ô! K�Š¥€É	C	Š	n
§pt£‰É‹ ˆ L!£!ð"! « êjÀ´D"	:	a	Ñ	v…É^¦B|�©Ã`8ò-3  * é"+#ä# •.›�IV	o	¦	Ç	ú	—
³ñÚôˆ¹wí` ' ¯ |ëJz(7¢PµÊÔ�™ÄfFË÷�[_®D" x U•áï–(ºIão}
5ýri®2 Û!"V"Ê# I ,mx°ñ	
D
H
¶
à
\£íôóLQæÖkØê×˜ !y!}!@# ^g³	¬ÓØE~·ƒÊ÷Í„ªð­¥^ŽÆ	!Ç!|"�#¾# k
d^ì(Csý*ŸœI5N[D;5"k"ü";#W# ¦f‹ø-	€	×	:
Â
^¾W1w¿“× �³ojE Ž !j#!$ )>¸
x¯ø)
£
ð^»1ôbl:û:" +J˜‡/E”¿DŒÌ­«ÞëPý6#) Y À  Cq¹î	
Œ
Õ)FU[y§?ƒO× #ñÿÇYÆ¸¥  s!h"á"ô"s#�# Ö#^ƒ?;kŒ’˜‹f´¥©Ü‹s."•"$)$ % d‰¸
“
M‹ƒø<Ë&qëä/p¡;õ!J!¶"U#È#/  # d ñ ó À	„ÝMv²—â$P–¸Ó%GIi³ìýÕ?AÎçôý-5Åb¿H!µ!c"Ç"
#$7 9 ] ßºõÊÞ:Jqt†»¾Á›PS	Ä	÷	­
Õ ûü9@UTXœ &º"é’¤úÔ‡Ð˜± °!·!e"÷"$$6  f k õ 0CY­ÆPšµ)ßîçðk„
5§ÈgÁŸb{ªÃr8½pÇÜþÐ-3QWdŒJ Z!Š# {Úaåÿš	ùÀ�¬çgk¯³˜¾0>ö\Auƒ‚ " @ ‰ t°{_N
Q˜(¿ OÎ�½Øµÿí†Šµ£²¤ùð ÷!‚"#: <  ¿”Íg>~ÙÝ
Q­°Bc«Ì¤ÔsvÄñ±k¸økn~¥·•½�u’� ÏÓÞh S!!"$"p"«#ø# Û 3IÑÔvÝ	vð	
@C^ƒæÄwñ 	ál!�!"¼"+ ’   FWdÿƒYËd«„ÚÿZly‹n‰•7âé0ANýDf›¢è„V!ª!Ž"Î"## | § ù+ê	9M²
ÊCL”-/†S$yºrqC;t‚øqÁ	"f#¶#$* + ×¼xüGÔ×,2¶ÅÈÑRiˆ�µÕ'BK™[ZÚàKbW(>í�!Õ!$$( ì 2›¡	j	Ô

´ w�3è'žÝäù÷IÇ¢™)YÓ »* à!'"É"E#†#0  `Ÿô#L[ëlÔ•	Ê
5;f™ËWÒv^b¾&7]e6Eãé‚Óac
Jt ü!&#x#’#¤#¯#ì#A 	 tÂ×ÐàîJ	s	‘	¡	y
EqTÏÕÛ8ly˜ÑÝæòüŽ’ÞçSk§ËÙÝîÐgô=^‹” ªµ)±¾é/7ÙíGÞÆ" ‰"& :ÊÛžÌÞÑ�É:R	h8�•ˆ©ü8³u´Á$‹½N×"ù"##m#) Ó h§æ÷O+h~“ÑÔUv/_Ý">I^¸»3Uã­ùÆë&ÕÝ³Ë -!#,$ G �kì¡
obƒ|�;£­p Ò ¡!ç") ‡ � «G™þ'ŸÛb™Õ‘
ý'>JV’ž*0XdjÉ=Ú�ØÞ¯£H Þ!a"ß"*  ká�äRTam‘—-2<>Gµ“òôöü¯
þ
f(nzáãéÖ­*Õ0ŽŠ!²!#3 mLx¦Ôòû14ê/@Ã·áóørãæé5Yî
’â	W®Z¢K¯ÔùyåPo�!!ì!Â"Û"' ã’V~;o�×%’Å
?8•|!ŒÂ8šÜ|Ž—Ì c l « Ø &!9!¹!«"% ²›'vˆ¨É¤µøþapÐ
ñ
I×"Up£aC€× …áÿ˜_ ‘ !N"' _ ñam©¼í4L¸­ŒM^	º
a	þQ8KW“åÿ0˜Î©š¬Ï!"*"ó#$$% Œj-i¿L¤\^	9	U	n	¥	Æ	ù	²Ùù]¥N‡T¦©Âü,2ˆ_Š$ w Tàyw•²C
'[|¢ì}¶‚É	4ö¡É¬E¤Êœ×­1 — !Ú!U" (=¥÷Ö	(
V.“Cv‹¾ËH�4üiD 4"5# $3  X c ¿ ð ÕBcp¸ˆ
ŠÔ‚L;±px¦$F‚ëüÿ¨ðü,4Å:ô!r!´!g"”"µ"Æ"ó"	#T#$$($2 Xs¹ÉO™´Ipsz½Àäïj¬
ú4TŒžSfz›®Â¼£/‰ø2tP � ¯!ö!‰#6 ; { Ú ø*VéÐ‚X8Êª±
ƒïÉAbªË£ÓBðBx#¤q”@üvðœstàƒŒ!�"»"#÷# "K ç�æÊ¡5Ò)ì! Ô!ˆ"w#
$, g�ªÚN˜ýg}&.Üë9na‚7=]¨·:Èâ¬ø�³ÀM%ÜÊ ,! !`"Ö"Þ":  ¥3uŒ–§,1F$àò÷qåè4X~ð
7H‘”á{o ¢7®ÖÛÓ¬)�–Ëä/�× %!‰!ë!M" ‹ði£ì ]	m	'
UZë|µ-�øB\Š¤MSû‡¬– 3"0 W ï rJUÔoÈ�·yÉ©«
‚î‰Óù©ÊÒ°e­wŒwÉ£4?.ûrs(  € Å"ò": V qTÓÙ¤ft¢ÿ+E#-ö38W}�6��àízˆÒø¡Ad‰¬v÷6­²>Ò-$†Œr«É $!+!Ä"Ý"I op°56‹Œ�¨©ª¶sœ¡BCDTéõJ27‡ˆ”›S
f
¢€ßù0‡Ñõö÷tu,¬±šg°±²lmn€�—"#‹qµ È ò #!`!a!b!c!€#�#, ±7Ž«±	ù	…¹8K�•œËŒ	T
g
†
UŽú1-­›Ö )yo‚˜¿âìä[ ó À!  Ö ß·ô�Uêx‰E	£ô›«ÛçrTh³{2f¶ \!d!"#‚#$ 6 q #ŽsúVÌþ0	ƒ	�	
=
h
‡
L×Æä*s…×]ØßpÀñR$S"  2Q¬
GúR‚Ït�à[gû	2;Û*¥
™ãí•\ Á!0 m ¸ É ÷ Ó8z�²äñ
¨ÈºÁÛ'9L�Ž–�0;V�û:rº.ŽkÇ!Ä$ƒŽ¨’‘Ã  ²ä†šãU
rél®úœ~zåæ ô N!"" h MÖä	Y
õ%ƒsíªÞÒXˆŸ|çÌ3\W ü t"˜"##ƒ#Œ#$- Æ × E[¯Ç¸õžÅá5�¾þ05mŠp
·œÜ-èÃä,Y¿Ç$'}”9Â²ôYŽ]!#3 6Œ6ðV+yÁF	s
¤B¬·½ó©iÞ×ìJS vËîU…šy´·5¾gnvL Š · “!¥!î!¦"  û Ë>àRëÂ¾
Y™€}År (i.Ë¥äe!à#1 H¿X†Â€©Í1	Ž	²	æ	
>
ˆ
AM±Î—åB•½Â.ù¼éšÄ’´ºÙàÃ€÷TŸ P |#  Þ ä QÛ�ûWÿ„	ª	ñû:Æ€ÀÃ†Øqü/òõS…©%T ì ¿" r ­ $	i
GÇ+Na±^jÁð¼# 7 ž .^}th	Ÿ	ì	tØ›6`i¨±$	t�Ò�–Hd# ½k�S¶ZuŠ‘á\h		
ì
É.¦ß–] n S"3#% Ä R¯Eq÷ýƒ3HTe§¿ü©
»«<É+K£°©âä 1"^"Þ# å­»CÙõûF¡f	§
ï-ôÜšî  ¢   3þH'Ðt{gË3gäC-¤ »!Â!"­"ü#$ ¹ ƒÃÔÜçZå¬×Ü(:Aò'	z	4
~
‡;:†F÷e"Û „ ­ + 3 n „ ç ø ¤l{³@ò©»ÂMª‚�œ	Ó	à	<']¶s»'»:øºœ“k’} æ#' Ê ì9ÉC[”ž<	!
W�”‹ /›Å"2ÅÎ”GR[y„�©ÒÚ<^± ½ Ä [# / �ÚP£—1Ÿü FD�ÐlÈ¾@®ô%>è5MJÕ# š€ä­$£Ú	Ç
sxŸ®/bþ4ŽX»{Ö³Ð, e  ø.=c2›Z	c	þ	V
ìÏê*äö·Ý~ÞZg™þ (!†! ³Ãç‡Ë	/m»TmµNœÞ�¶b4½wæ5 Î ç 0!O!-# ”å×
æ~XÆ# áð¯û~z�ŽôÎÚ õ ;!"ë"Í#" ´˜Æ@	‡	
Z
™
}ö=Òht¤õd¾6i’ML ã|> u"š# B N—È× å„"a«í¨JâEÓ¦Í47E; X P"™"#$ ö	+	~	Ï	8
nCî«�ß©ÜéY\Êõè]ý !  i ê ó0’	q	‚
Ò
Û
&K»à"g×\iü=å‰ }&Ì"$#„#�#" E Ñ Ø °È)J²�âõ6�³Óú Kn åÀsÈÃmˆa †"³"å"#? Ç eŠŸâ¦Æ¿Çÿ6v…‹’q
SF©µÄÙÝ.WjÏéózmÐÜâë *~öŠ*Œ�Ù
'V%~•¦ÊòZ�^!! F\½ ¹ö1cr½X	¨	É	ü	y�Í“»Ä-ºü2Œæ:±õ "º# — Ï xžÅÒó
¸§Âh¹ŽZç#(E³@9 „" "! Q ÏC&dG	"�ª>ïˆ»ï-;Ga¸µoš y ‹ !¦!Ä!§" 7�?}zš¥Á*C¸jßN„ÕK!wÁGhwM & ™ Î7ƒ,Â 	N	·	¿	_
ºÄþô%²1‰¥Ìïz%µ´ûV¿Ãz¹¦ ¸ æ!O#" € 5„úñWÔ
t
ù
­¾5àØíTÌnV†›*6b~œ ”!ï!I"$  v­Ý¿
õ
ßIÄšÑƒéö
Úú�–°ó·Øåi!ù!?"Y"¢"á#é#þ#$1 á Ìázª#aì§ã	P
$RZ�ÁèQKQ�~Æ}d‚f¦/B¶“ ï f!o!�!½!ã!" Ë)£»­	æ
¨` ñ‡cs·Êy¥"œ"¯"H#) ‹ ü ?�S·ÃÎè/
ˆi¯ßër�\�¥!¡¿ØÃå)‡ ju«/Ìu!* &Y‡ÃNdl�û>F¯C®2	³	
?
‰
�
N²#Ê@ƒWñcáÐŸ«øø ? S µ ˆÀÅ­Îç Qp|Œ—¦ª
$ï6		
Í
$cŸ˜æÃeÖÆê'>†aaÅ:^$dpÍh½—ù!ºœ éQ F"‘"}#Û#+ I…“ˆ(6²*Bgç	BÏ–/P\”½îº@³ç	•ûrJ	Äg�YÕ † w!)#2 ) > 1¾é<Î×Q	�	˜	Â	õ	L
²=C¾ÙúY›³Ñõ“Îâµo»InŸÚ°�UqÑ¨!","¢#­#, a u ½ R _+ÜÏÙ Ç2Šœ@S¤Ä
Içî2rý0Óö]ªòj  !!7!©"¹"À"R#ñ#õ#&$! ÑÑïÞüŽ¼
9lòS_
³—¦4ÙÖ „”"&U Õ r" å ÁXê	Ere‰¸ÁÝEwó®à{ ¯ â b#Ó#ú#. ß »‚=�–Ûô 	)	|	…	«	
6
€
ä
ü;Ž­æïÏ�ÁÕ<úŠ§‡l¿_ó$T†È>í M#& s ± ³ » oq~€«\^i"@%,.<„>2bÌêñÁY[hj•HÂ`#Ù# %:é€`	–
È¦ðó{¨—ì_íñMÑ!ó! ® {î]ê	G
ß
6H«ÒnDƒOó€˜ÃeòÕj|!Æ!"½# ®j
*]½Ic†0ºÖ›²OkC� 9":# ]í	–Ô"ÍÄX·a¾G!Ç#& 8 /BzÞ`9ºí^O—¾û?aj©²%q½
ìÏù=Ó†£—ï°
 Y!�"/ Ÿ EcÌLfu}uÙP¬¯ ruÃ.Rm‚ˆ·÷m¹åáè/Mšp‘œÝR!U!"#"e#ª#µ#( _~žÖóZskI	i	 	pSœÎÔ7xR¦ÑuHafÆD](VbŸIs €!&" † ¦9T^j˜	�
û7i#Š¼¬o Ñ Ý! �K}Óñ:l‡�’â`í
ý
>™ÿyà—^ ª  !$ '<l¤¾·x[v‹]	Õ	¹
u½ÊPVŽ¨«Dþ/—Í0 Î!Ù!T"4# z W÷¸ANi
 ¢u§ï3O‚Ó!õ! ŽïIn—¦p¨ª
~3úLÊŒ  2"_"ñ"% S°µrøþ,„I1|ÊD	‹	��q¬«=,xkáŠ ªã´ Ç  5 p "ãF�ÎÚUfýKqrÆÃ}¤	”Â å M!"& Å ú L5‹½/4*À	o
¼ò¨ÖëIuÊ„™|‡“ž¤±ãm‰ û ¤!ß# � ¬ Ý -ÁÚ	g	ž	±	Íð_§#M`‘ÂÑï.•O ¾"{# ®¼BDôöüµEG‰¦
¨
,ºÊóªÈ -J¡ £ 2#* £æëkY«ÖO¢;	y	Ò	ß	“†EC…Eº½ÄöÍó9=d›ÑÙ4L!IjÚå# ¬#¢b	å.w)õ}Ýafy3ó + 4 ê",#Ì#  é –/Å?	p	Î	˜
|£ôBº~[hè<Ÿ{™#, – dÿIœÄÑJu„‘¼W	§	È	û	xE´Ì¸ÎòlÛáõ‰)Œ	U"¥å°D8 ÿ!Ÿ"²" 4‚¾	œÃ4îaÂy}› ¥ Ã! (â	‡§hQ_P†|×xt.n!�!¼!"">"X"®"è#ý#" ( „ÄæckEo{–	);fB­P	Á	ô	K
<`‚VøfžX… E"�"" ^Ð<ØÝó(	{	
5

ã
8^ˆ	Ÿ£;H·òDÒ#\® á 6!Q#ð# ° º 9è}[\­!F
)5¼m¥ï1z§ËÕšëgñGNÔ{!_#( AÕÝYËìK]|–pQ‡¶öGeÅçCÎø…Ì'UWaoî`¯Ü	 X!!©#´# y … V¥£|ð9†—Ô	¸
=6t¼Ÿþ�‰–»«© Ü!, 4 o ù !îHm–´â¼ÍÙ.o)0JŒp~ŽHq}ª2|ùj{†’�ÿl“~ ³ Á Æ ú % œ ,¢AóªN«¡�	°	’Ìä("Á¹¼ÇõÿeÌÐIØK”Ù3 1#Ë#$ • è cÃƒ�»Ä>	á	†P^·{(Z‹;Tsž¤¯zÁ|š "="W"˜#ç#) ;[ÊD\• {ó	
E
J
â
•]¤î_o0yõUæMñCTÓV`níà z!~!^#¨#³#ï#. ” › í¢…-n/ =	·
‘�O!YpÀ|©ŒÆôËÏSˆ•�Hz…‘ª×þØ™ ¨ ² À Å 0#—#R ¡:yz.Ÿò	I
á
XÜ ŒN\]^nXœ§¨äå‹3456ÆÇÈÉÊ
RçèBS�¶ÛÜSTU_lmÙëýêëì¾ ¿ Þ ß !?!@!A!B!˜!™!\#]#•#–#§#²#( J Ë …±"	¹	"
a
YÆÝ2u—�)39¯#7•@FShé\·Ý=Úì!C!š!A#Â# ‘Û	ýv„ËÎ…4ð4:m&–A_ê]¸Û!}"B#& 0 Ì f$ÆQ¤²˜Ô3öF¸*Z´�˜ÉP®¿$8Çé6º ¬!è!  h†2ZÇ œ'W{EžÑâA¯GT?ÞKÅ»3!D!È!Ë!¿#Ö#. K ¢ _´		#	º	#
b
­ÞÙøG«ñZ‹*:�°+¦õÂJOi�>Nfí†Ê
!›!Ð"#ž#Ã# ü’%£cä8)5�þˆ{�ñJtæ|7^¹8ÑÃl"ý"<# ûð
¤y¯ew_… ¯0íÿ/t n¨´X# © ›®l
´
tãÌ+6O\- !6"~"h#% �ÜåÛ	È
þEœÃÏD†5Y¼5;'€—¼BW`×ëEÜ<þf "C#$ ™§gù%/Ç³Ã
_€2xÀØ!Aƒ‘ÀßšNF � !­!k#"$- OgdŒ3™ù.	[	�	ÿ	;
W
Õ4Xë÷+å+[µp¸ø9Q[p,ê7À)!‡!é!K" 1 ‚ >YRd	Ø	í¹÷™V¯´Xhúÿ ž » ê  ~ Í  óœ¥
 ¿ÀÐG¸Þ Ž”Ê%9EÈsdkw ¸#( ¹/y†°èùOŠ[n!¼>_"Òã ßùcpmìÆ¼çE!P!ñ!"K#×# ¶W‡Ì	¤
û
Ìñ| Bsv;ß€6 Ï Ì!  *7?Äi3Ê0�(UXF¾)2�·cH@x1!4!–!É!;".#À#  -´ìîˆ*
È¡ân¶Ÿ¼Oõ÷ž°U5¾Lüè  £ µv
çÚˆ0$•Úïùñf+‘,ˆ�§²�õ?Ëœ!ì" $	$
,
c
Ø
é
®7FH±üQöþî!#! æ`;Ö»	K™YÇÀÎò[U¡¤°&¬ß=PgÏÛ <!"7#Ÿ#Ä#*  L • ¾
	,ßEN�–Íâ®¬1vŒ;AìQ`Î{��ÃKj„O‡& ö Ñ"Î# Z µr
[
>*KÙ*zM-žØ}º*? A"m"v"=#   Á D~é¤ÜáóGk5PKÈ}Ò¦ƒ!["â"þ"t# “™ &ÇA	ˆ	ï	
�
š
Ö“V\€¨Îuä8Ç9ï t!i"î"›#1 ¥ ý!º÷ÓiuŠ¥Æöe¿7djå96N�¯Üÿ@„‰M|ò$çò d¡_Z¹ŠÄÍõ"�#! _Çz°<`�“z†¡1¬î.… ŠŸD€¡FoÜ§8#$$*$ ÉÅ×Úx…;uªõÔªÝŒµâÚtY ž!–"¤"Ô"$ - O�˜ü„Ø¥@™µî¹’YÎF/"Q"Y#& C O ñ$¡Ñæ÷
fl#bŒ°þIg|¦©Ó0K›ãñ©5< š"$ xœ
”
NŒ„Òäù=pÍì,àå“]A<öþ 7""·" ª 	Ð	m
uo¸�7¢Ë) . !K!×!ã# & ¯¹µ
âÌÓPq]öé^!É# weŠ÷,		9
Á
Dï¬ë'rü)’žªÝê0aMZn!V#i#% " $ ò 
‚·Lu³¤Êá#%ET•·êHh²Ö.>@æþŠÆÿI!Ž#%  e j ô ÙôÝ¯…Þæƒ
'Nœ¼—¹×o´Æèþý´Ïõ@c¶!d"#$$ ¾“Ü	Ü
	]j6jB:C¡÷~çÀg k!"Í" $Y   ë Á1Óßí	r	”	x
É
Ó
ÿ2w&F—ÓÖÐÜåñû4˜�ÄÇÐÜãøQh‡Ô&JVj˜ØÜíZ]½6<\d~Š“Ÿ©´â(�˜½è.6XaØìFÝ'=	û!È"%#D#…#ë#$! F � Ò Éö�Ë*užœÔQ!üHIU€‘�²Ùt²G æ"ø"##l#- ±àšãú&0È;£Â‘´´ûo®
Ä
Ö!TVY¡'JÁB„àÕøxOn b � !±!¸!  ^ ¨»hßKKöLT	¤	Å	ø	`ý’3õ’¥Á£É›h‰™")"#$0 : Ù ³r‡¼¿ã7î�æ:ASRyšÁ»#ê"þ“ûˆ›Ñ+Ä q!®!f"‡"Œ"´"ˆ#	$$3 î fhÇ|‹¶%Èñ \	l	&
GYê6{¨É,\n¶Ñ÷‹9áÈŽÚRq.• ˆ!ê!L"4  4e› *è7w†Œ“šR
e
…
T5ªÚÞìø/Ðæô c«s+Ñ(S¯–ë1eñ *![!_!¿!&  l È ö 1Pã�§ÇÀú	/	‚	 
<
/ÖÅ9kq¹�ù„:\#§ðR�6 g DZ®Qàï4ÁlãX
r
¶¶,X‚hÂÝãì+©|Ä¾qÝÿÑWxÆ&-8Á4½ËóX[�K ’!‹#& ã G]|¾©	å	°ú”¼Á5ø»èh°™¿€³°i¹û„¨ö ë »# º2Sds¾	e	ë
zîÈB¢,Ÿ " 2 ƒ Û?Z©ñ&	›	3
}
ž&\ÿš1?­÷ç;] ƒ ¼ # ÷bæ Y	Ê	Ù	ý	Ö
ÎWºSž­Å.al´àý3WµYŽ�Õ²v A Ÿ
Ú
Ñ!‘ß!fá K[É ¥D: Ë"# Î Ð ±ô�ÆR¨ÁÃi’ºyÏê)¹æû&‹lñƒ"…"¹#$  ˜ ÎB|cÓM	!™¹À)Mƒßî‡¤Ë,:F$)³úF´¸ x !å!. Š uy¬"`¦º.
O
ô
HŸÀÃÞêP�[Ž ¾è	Ùù�•¶É¶¤teAh!â!ø!¡"G#G = R %0‡'èÍ±ú=P‹¥Ö—	Ì
#ž±"[dØ“ÉÕÅ`²Ðô]¹#?²oÌg¼ðúqnHbmž €¹p›ÐÔè §!(#¡#¬#) ºÐÀ•ÎÚR¬²î¥‰›?Ôù‰¦	¾íÕÜv
“Ç!ßi z !q"¨"L#Ò#ù#  zíéh?Þ
\ªHbCò$¹ðÀ–dìiLŒ Å!ò!"9#Ø#$ DeH	o«®½ÍtwÂl`l¸Óä!à.<ž¶½F!Q!T!"""%"Æ# ö8Ž·@JÒwü
h"x¡Ct2�Ð Í! 4+{ñÕêÅƒ˜Âwàâ" Ü X~Õˆx	Þ	¥
+¹òDL_„Dxò,2<cÐ
HiN ½"z# ½	Í	w›¦ÂDÍ
¢óAkàO…Ö}ç!x7 m!Ž!þ!ž"±"# 8@X]Ï bjne¬O	À	4»lž¦dêBGÍð„&¶ÑW5!W!�"P#  +b¡G8ÌØÃ¯	…‹Ë zGŠž1¸þd{£JyÀ{ù <": “ š  „Z„-!	¸	`
ÅÛÿ1Mt[mWox–¿(28®T{¦ãŠ	”?ERg‡œðAµRüé§ Ý >!—!/#”#¦#±#Á#î#* ¡ e#Åõ›&)VY³YzŒŠáï
*39OÁINeÄ…ºÉ¹ 2!«!Ê!ç!Ï"# ¨ úïû³
¢®âDã7›Â.‡z»såV§67Âý"g#$ } � ÿ N˜òX
¿o·U÷@8‚WD+ùr¿Mcv � é J"·#'  6,.…µëíVN‰ú
ÉË á=!½(öøoŸrëu•!ð!
"J#   
:Õ½+
u
è
6M•ÙîÍ0euT £%@P_Í‡œ±<ƒ%  ¤ èÛàJ‰ÅØòj®Û4LÍ,c)‰Ìî‚!@"Z"í"& , N €ÄÆÐÙö
:yýH{-„Ò‰ž
Cš©ðôÛ¸‘XáÙ�!£"Ó"$$ wvÀ
áÑo·êû(‘ŽÒ’`Lm@( !Ö!â#F  ! Ø®½¶ÒÞì“	w
1t›%–ÒÕÏÛäðú3£ÆÉÛ÷S”¶ÖéUgn—±ÅÛç\i-[c}‰’ž¨³á³9ö?æj!ú!ê#ÿ#$' Ž âœ¢í£	SGR‘HQ�™ôI±ý¢÷š*wg p!"("‹"#‡#!  í 3{$Àâ	k	%
Q
d
.µ8�¨àƒÐR"wÅ®ï¼0” ð ‘!¾!$ â ¨ð	%	ä	2
|
Õ
ê
¯%[V4R`Çßþ
~²�¬¸Ô¡§+0 ÍA«!b¹L	-
Ù
‘xžÂÝéé(L‚�ŸÞåúeøàJÈ£+ZÿC·!g!á!ä!Ê"F#1 $çìÌ<¤Õ–	Ë
©GaZ±×í’ÈˆšÁ#Ïó\¸Ó±_¿ËflÔëÛ	hK"'#8# #Å#Ñ# õ*w	¼	¡òÔégC‚—+11bÏý!�"°"y#4 a \Ÿ"am×®	ŠkLsšXyVw¥'c­àî	8SzF‰ƒ“À>MQ†ïIQdÐÈÜ ø =!“#¥#°#í#   þ M—U¼ç
á6<šÁÌdt ¢?ö'?^7�äê¦$ u "I#4   M uÃÅÏØÑë’	àÐ9snzÎã¢Ú“­è	3Tm¨ÄÌÚæï�Ñhb_|‘²8làˆ' !Ò". 
 � @ ¸¿áï	K	¢	1
{
-€UÜ_�§GP�˜Þóý	�°ßßÏ*Œ¡«·!vÄ­vŠ"2 ý –ž!Tv	‰jFr°Öàì‘™"ÓèÀx>Bíõ>R€ˆ�–*k’¿ê0L…Úî0ÎHPÇ# ÷ Ð#,  Œ Äéêt	u	0
z
]^m¦FOŽ—Òç¯Ù2lwÞ<=a€•¶�‘k¬Íß!Ï#  4,  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                      
                                                                                                                                 ?                                                                                                                                                          	                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
                                        	   	      !                           *                                             	                                          1                                                              	                                                              
                                           "         #      
         
                     ?   ,            	            
                     	   	                              
                                 %   F   *      6   /      (   /      &   Ÿ   n      &   0       )   ,   y                                                                                                     
                                                             ?                                                                          	                                                                                                                                                                                                                                                    
              	 	  !         *               	              1                      	                      
               "   #  
   
       ? ,    	    
       	 	          
           % F *  6 /  ( /  & Ÿ n  & 0   ) , y   �L  ‘È  ‘ê  ’.  ˜  žd  ¦è�ôN#¶É! €ÃÌáÑtÒ“Û2Òh­	³ê0Ö!í"“#¥#= cHÞ�X úã7"7§Šæ”f“"3u b#ÐJ	‘	2RÕàÓÆ¢å/Ý `äúåbªµÌ	 ] ‰ ì [	°{ÔåçÐJ	j	‘	î	
Ô
	ƒ¿ÌøbªÝ,2ŽÈ$R±ÕÞBµ¾ä^b¾&E‹ÚàµÓôö ;YÆJ!†#ø# 
§UÛ?“ò
¦r= “Ï
ó= “= cå	HÞ�;¢AÏH�Ïïœ#¶={û!1ßè‰äèÿïç¹ó×±ÿï…!çó­ÍˆšÍ •Üó~·ËÌË
\
-ÏÃ
JË•Ö Â/!°F[+F[ËÇÿIdÉõ*	}	7
ªœ¨Û_ÉdçÚñÍav_ˆ ¿M
QOÒP¤Ïv¢q 9öÿ#¾ ¥�ç\) º²uú›³õ@I¹7Ž•œUŽú-j ‚�R²¥“#Å«!¿õ*	}	7
Ñ
ªOÒ œÖ¨Û%G1Kº#)è°
È‹-ëp¢÷€"ë¢€"Å®3 n ’gvrORÓÌQ¥ë„\¢€"²õþ)è°
È+åwp€£¥#â¿OÒï
Õ1{g¯òé"eïa ¢	�ó¡ˆ–,ìLò!±" ¿OÒ$ë,4Æ"šÜˆ <¿ËÌM
×QkþK“O©ÒäïP¤Ïv&��¢Éê q u#œ#ÍÌ
#ÕÅ]è
]žGÑ5!P#J"˜¹Î	óú`Î	É� $œ{$a<mÆä	¯JÖy‘ÈG�
-6g�°±Sà2LJ’²¸Ûäk#r>çÚßñY"ì"QÛ ÄÚbíñ 	G?åÐÓ=TÅ º@P˜Š"°8¹Nú>½˜º@ñ¹9Îi"$¸Ï
ä	¯
²¸�)rè°
OÈRÓÌ‹+å-Q¥ëw„p\€¢÷€"ðóúê º#É{$amä	¯
gà²¸( •0«(< Y ñ {Wÿ	ÆµÐ‘	
Mû§Èø<CTgœ¯’±çGSÇÙ^gºA?™�±)7bÏV!†#«#$ä	¯
�SJ’²¸(
\{g¯º#Î	’|!þLšÜ|»ÌÌ Ø .!9!ƒ?˜Ù	WÕ] ºJü˜Bc,:´S/�©þ² Å N¤à"r#v
0$•Úïùˆ�§²)è°
ÈpÍÌ
#R²¥ÕÅ]“è#Å
«!r¼¿SRš®!e«®tÂlQ!""ji±	e	î(d
U�É©‚w?(_C	•
»
ÉdEp–
ëÆý¢˜ €"L î�OŠû
ç�ÚãÌHñ_"ö\úçê 4!‡!é!K"Ñ"ã9KzI÷D"ü"b: ½Siþ“›Äq!f"x,ˆ�BÚûã7‡zåÂ®: “›dG]®ž1GÑ5!J"P#™í‚ò×$Žt78Y2	Ÿ€U	¥	Æ	ù	²ðÙó‡ì_ ¤1ûgû
,0êâÛþL»üÌ .!„!“}"ÿï}ÍÙ :!Ž $œçÌ<¤Õ–	Ë
Z×G’ÈH�±Ïó\¸±Ëfl#'# #
)
»ôû÷ýÏ
`þ9 q†»¾­Ì9@svº"ékk·’ú‡Ð� S!!"$"e"$$ÌÖÏ�	D—ÝÚk¾´ôb‘Ý®ÏóX°ÑÖ´Å JØ­3LÌ,)ˆ@")è	Cjpc0}•¿‚ðÓ ¥GŽ”sdÌ—Ý¾ôb®Ö´9 q†»¾9@º"é’ú‡Ð e"$$­Ìsvkk·�S!!"$"Õ
VR`ß�ÔW…¨@–A-ÃÂž öÒht¤õd¾6iML ¶'KÕ!9 × ¹¼q†»¾­Ì9@svÔ2ÅÈRµºÕ"?ƒék[k·#Z’úà‡ÐñÿY>�¸ S!!"$"e"ô"�#$$$Ž $œçÌ<¤Õ–	Ë
JZ×G’ÈØH�±­Ïó3L\¸Ì±,Ëfl)ˆ@"#'# #aåÿ�¬k³uë	sš�Gc#Q &"�>»ïGa¸oš ¦!£	‘ô¢g"("§Ä,~�R Ö
WÅàŽ�˜@‚MÎ‰›?	íi !¨"Ëß°Ë	´	\
.Ù¸â+DŒÌ­ëvJ˜õKÆ@	‡	™
¿
’éú�ïÈãˆ‹ Ä!š#á#˜8Î‰›?@ž	‚íMi !¨"^ ¨»KL`ý’™")"#$ÎBc!î‡,:F´ x !ò‘�
Ñ
JÖûä%d‰&q/!U#  Ñë’	sÎã¢Ú“èTmÄÚæhb|‘²8Y q)y×. ƒùÙ™â
ø
èÏGm3ûC H"Ô#$ñlbg¦©ë'Krü)›žMZ5< V#¹™ù%/Ç³Ã
£>AJƒßì�NÆÑ� !œ!ý"K#"šXyàî	8ÀMdÈ©MüÛêm`<§Ç«¿ÛŸ!Õ"K�Šº	n
¾�§åÎ“kt^r®j=ˆ £!"V" 2•0ßäçï
“Õ«(•ãÖ Ä(UX)2H1!É!G3ûC $ƒù
ø
mH"�¾�åÎ“k^j=. Ù™âèÏÔ#× ¹¼Ô2ÅÈRµÕ?ƒ[#ZàñÿY>¸ô"�#$^ ÎB¨»KcL!`ýî‡,’:F™´ x !")"#$ûñØ%T 0 Q¸˜®º }|‘?" $ ò 
‚·Lu³¤Êá#%ET•·êHh²Ö.>@æþŠÆÿI!Ž# ! Ø®½¶ÒÞì“	w
1t›%–ÒÕÏÛäðú3£ÆÉÛ÷S”¶ÖéUgn—±ÅÛç\i-[c}‰’ž¨³á³9ö?æj!ú!ê#ÿ#$Y À  Cq¹î	
Œ
Õ)FU[y§?ƒO× #ñÿÇYÆ¸¥  s!h"á"ô"s#�#1$•.›�IV	o	¦	Ç	ú	—
³ñÚôˆ¹wí`; { Ú ø*VéÐ‚X8Êª±
ƒïÉAbªË£ÓBðBx#¤q”@üvðœstàƒŒ!�"»"#÷# # d ñ ó À	„ÝMv²—â$P–¸Ó%GIi³ìýÕ?AÎçôý-5Åb¿H!µ!c"Ç"
#$ö8Ž·@JÒwü
h"x¡Ct2�Ð Í!™“-<Â1Æ
ë}ã"¶Üÿï�M›Ý}º˜¼ÍÏýd Í Ù '!/!:!…!* šê» ÖÏ�		³DvÚûk+1:eµ‘œ´ÊÝöAÏó%YÙßèJ°Ò`ºÅ ß!‘#£#®#¤ èÛàJ‰ÅØòj®Û4LÍ,c)‰Ìî‚!@"Z"í"ã G]|¾©	å	°ú”¼Á5ø»èh°™¿€³°i¹û„¨ö ë »#* ; { ‘ ¦ Ú ø"*2KVéþHšÐêÿ‚»X Ó8Ê=cÖªÏØ�	±
 	ƒ³ïDÉþAbvªËÚ?£ÓçûBkðK“+1:BYekx�µ,‘œ°´ÊÝæöA…ŠÊ”Ï#x}¤¶ó%Yq”¡Ã5@üpv¼ÙßèðJœ°Òes�Ò`tºà)ƒÅÎÒì ! Œ!©!Ô!ß! "o"ˆ"�"»"#w#‘#£#®#÷#
$� ¬ Ý ã -G]|¾®º¼ÁBDÚôöüµ2EGS‰ds¾		e	g	ž	©	±	å	¦
¨
ë
z°Íîðú,ºÊ”¼Á5óø»è_h§°È#™¿M`€‘ªÂ³°È i¹Ñ-Jïû.B„•¢¨ö ,O Ÿ ¡ £ ë "¾"2#{#»#ö	+	~	Ï	8
nCî«�ß©ÜéY\Êõè]ý 8 /BzÞ`9ºí^O—¾û?aj©²%q½
ìÏù=Ó†£—ï°
 Y!�"m ¸ É ÷ Ó8z�²äñ
¨ÈºÁÛ'9L�Ž–�0;V�û:rº.ŽkÇ!Ä$ƒŽ¨’‘Ã  þ M—U¼ç
á6<šÁÌdt ¢?ö'?^7�äê¦$ u "I#åF’ÐÓÝÐÚaÈ 
g&=Ž”{‡º2)/TWc<¢Åê×Ý®Ô¢#+$4 o ù !îHm–´â¼ÍÙ.o)0JŒp~ŽHq}ª2|ùj{†’�ÿl“~ ³ Á Æ ú ; G { Ú �ø*VéÐ‚ËX)8£Êk»ìªö	+	~	­	Ï	8
¡
±
æ
oƒ¨bƒï`ÉAbn ªËñC|£ÓîB‡ð�Bx«;cs�£·ß#¤©ÊÜéqy”@üvðYœ\s¥Êõt­èà]ƒp Ò ý Œ!¡!"�"œ"¯"»"ç"#H#÷#ûð
¤y¯ew_… ¯0íÿ/t n¨´X#  6í     ¶  Ž         ŽÁDÏÖsWÏ»üÌ ñ­¥�87
¿L!3\Ö–!);{iÿC©Þ¦:ðì˜¢ Z!0eÖ­Ýtæa§=LÍi·•uS�8ÔébK¦5žè’è×`—JMv>�AU7ÈØñ �IB	ÆÈúitp.� q}áhóhÑ¢jR>NÆvWîà¤}„øîàåäýã¥>ÖTèú:Ô’"?.(ˆ{‚^Doíê’Ò U»“H3ñt¡S_D»±Í°èáLÍÃQ@0Ç‚sÕZ¡äÁED`#2ÒiL E¤lÆUk
Pô TUY•¥]™&S@NEÆ³J[àø¤Wm8‘ÕžÓ�}¿­ÑEéi™gI5þæˆ�µA‘TwÔêGQ‘UÕIGß7ML<áºÑøA„ZR™•™RIU¡}¤MýõQH#DEUH�N¢¾€®€M4Þ`3Lµ‡8p�GZh¸&HÓMZŽ9¡×M¸£^Ý. ÛSNô5ø8�QŸ•×Nø¨ŽàUí¨ßxâ>�c’�%•»�tj·qøÑ¥Ö�Ôég{éhQlÕ}5E…ÔZh‘•€Ô¨�h*]—Vƒˆ&eZke†I:	
”Y*£‰êæHY�Ð­ö›*ÑýûõÿñÐüPYQBDGDtokH\±Âl)z¥QT  	Ò4Qd‘Ùd×GgW•�UÕ§U­V©±”è¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQßäNLfûyÕaÂeFØvÇ�A‡u$Ào’—2ÇÒâˆ‹J@ˆ/ô;ý44RcTØÛµ¤´×Ðˆ/ô;ý44RcTØÛµ¤´×Ù/Ïù{ÏÛóþ^óô¯?k¼ÿ`áåªªjcD<CÏ°û„æ½’Cª Cä:£UNŸañ'S÷Ï³ßÌqËýÉÍþÏ<ÊÏÏs=ß,oÏvÄÀ·ÄãQ{¯·3,Çl5€³3Ì Âà}ÏL±L Â+<FÃó6„Â¿3Mt`PûàÏ7ÃõüÈ+à÷Ë»?}P.LòÈïðHÃÄÌðüúøûÌÿ5ûþ¹ÄÃ°ë|·Ï±üP|Íø#aüÔü#áÏÏ÷Í3ññPLÜØÛ=,uµû�§U¨…F‡‚k¥æ(ïªs˜�œã©cê&ØæŽ¿&­ªï¾‰•¾
8»©„ˆ?(´5È2�â*8s_ýà £ß~-;êð¬êÿïæ�pÁ{lóãèüã�ÐÃŠxu¯ Š,N¾¯`ã³â(÷àkŒÀrúãœ¿¬?µ½0~¾¿0øãÃ¸Ãî¼Ë¬úÏíKïêÏ¿Ï�k°Ÿ©GÖ�CN$n“ÞªU@àP9k{õÅFãªŽ‰à.î
GÑ¸O€Nî^@_Þ:SíeN�¡#­I"#òâˆ‡¸%äFþÑþ©-"[¹4’JØ¶é¦´š™z5EafÑè©£N™`§VTÖM·V­Õ•6ª­ãk’ˆ*/W¶Ö&é¶.äæüÿ—¼ý¿?åï?Jóö»ÏöZª¦¦4CÄ<û¸NkÑI!:ª>@rZÏæHK&œÌíàòÊ§\²Wø2Ÿ×¥³èÓvÿêÏN–LQÍñ³úýt£4øX1`ÏâL3iLÃ ‚œÍ29Þ�LþLüï«:ºþ¾®Ïr³Ã«üµ;•°¿©¼jHêpß±ÿU^†�!‚·$€öÏá‘ÂªÏê KÜ®¥’º+Õà]wµþnÂæº¡©”¨ñ«š
UXN—Uj\Åª]Vis§Z�ƒz¬XŒÁæ¢¦ªågkVbo;aeþXëYf…TI„š×jò,þšþªŒ6¨úøªŽßÑ\à…ITc…J7�±xµÓ	Fú*³üQr«ÖMê•+ŽbÂi¿&‡<D×°³Í�æ»E‡ ¡'·ÒÝMºp`Øt”E¦4Ž=—ØHeÞ0õéë‡\l|PN_:ª7]€Ø—³èl Á8XSÙõqÄÈwÜcâM`×\£5y¥àÅç§¹:ü5Ö–G��R�Þ¸ÎÃúðàà©£†VàOL€»0›hÓxÑ‚Sûòl’›�Ç KC^`aqY8FB>ÞSh%âÑõ*{fg©•zÚ©jUä!Fú¨e»aõ[ú\„ªÊ÷„ËGü‘TmPnº¾ª®3%¸ã£WUWß9ôÂ
Š�ÔN}{ª¼P—ƒ­4LÑŸVx®¹"O$¤=÷­Eî$ß>v~Îã–ø§Ò8ôƒK"éð(Ôq’ŸPÍ0°8\ +#wOÓ L\~wÅáùÓ!9„TÏs¯Å	¼%0Ð,A7¯@sà›©’ø°*Ï~áèL±ªˆc²Ð Á>`UsÓ\Ãê§
ø>¨àú£·bº¾2›þâ_&Ÿ>¡!8gÎÏ4ñqñ3LKHLA  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  +u<?xml version="1.0"?><Features nextValidID="27"><Feature recentID="26" name="AmpR" directionality="1" translationMW="31558.16" type="CDS" allowSegmentOverlaps="0" cleavageArrows="6096" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="6028-6096" color="#ccffcc" type="standard" translated="1"/><Segment range="6097-6888" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="25" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3293-3892" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="24" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3959-4547" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7059-7647" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature recentID="22" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5442-5897" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature recentID="21" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2954-3283" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2234-2537" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1366-1599" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4634-4867" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="RSV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="294-520" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Rous sarcoma virus enhancer/promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2538-2741" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="521-701" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3134-3269" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5100-5235" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="748-873" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4939-5060" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2095-2211" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5923-6027" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="149-154" color="#ffffff" type="standard"/><Segment range="155-172" color="#ffffff" type="standard"/><Segment name="-10" range="173-179" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="113-134" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="T3 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="248-266" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T3 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5256-5274" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="187-203" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="M13 rev" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-227" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5284-5300" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4429" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="4046" maxFusedRunOn="4046" detectionMode="exactProteinMatch"><Segment range="4430-4441" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="0" name="3xFLAG" directionality="1" translationMW="2730.72" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2904" maxFusedRunOn="2904" detectionMode="exactProteinMatch"><Segment range="2836-2901" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;three tandem FLAGÂ® epitope tags, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>9d09dad2-79cd-4df1-af32-0dbde0e69422</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:9:52">2018.9.13</Created>
<LastModified UTC="0:56:58">2025.3.3</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   ©<StrandColors><TopStrand><ColorRange range="0..7821" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..7821" colors="black"/></BottomStrand></StrandColors>  8~     ¿  ±         ±ÁDÏÖsWÏ»üÌ ñ­¥�87
¿L!3\Ö–!);{iÿC©Þ¦:ðì˜¢ Z!0eÖ­Ýtæa§=LÍi·•uS�8ÔébK¦5žè’è×`—JMv>�AU7ÈØñ �IB	ÆÈúitp.� q}áhóhÑ¢jR>NÆvWîà¤}„øîàåäýã¥>ÖTèú:Ô’"?.(ˆ{‚^Doíê’Ò U»“H3ñt¡S_D»±Í°èáLÍÃQ@0Ç‚sÕZ¡äÁED`#2ÒiL E¤lÆUk
Pô TUY•¥]™&S@NEÆ³J[àø¤Wm8‘ÕžÓ�}¿­ÑEéi™gI5þæˆ�µA‘TwÔêGQ‘UÕIGß7ML<áºÑøA„ZR™•™RIU¡}¤MýõQH#DEUH�N¢¾€®€M4Þ`3Lµ‡8p�GZh¸&HÓMZŽ9¡×M¸£^Ý. ÛSNô5ø8�QŸ•×Nø¨ŽàUí¨ßxâ>�c’�%•»�tj·qøÑ¥Ö�Ôég{éhQlÕ}5E…ÔZh‘•€Ô¨�h*]—Vƒˆ&eZke†I:	
”Y*£‰êæHY�Ð­ö›*ÑýûõÿñÐüPYQBDGDtokH\±Âl)z¥QT  	Ò4Qd‘Ùd×GgW•�UÕ§U­V©±”è¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQßäNLfôÝ ÃÎÄ¹?ìAçT}‡i•aÛuaÔ“¼J\ËH,ˆ"-) T¿ÐïôÐÑI�ScnÖ’Ó_B T¿ÐïôÐÑI�ScnÖ’Ó_d¿?åï?oÏù{ÏÒ¼ý®óýƒ‡–ª©©�ñ>ÃîšôRHAª��ê�U:}‡Ä�OÜ#>Ï1Ç/ô'7û<ó+<yÿ=Ì÷|±¼=Ûß�Eî¾ÜÌ°;°Ô"ÌpÏ0ƒ�÷<2Å0¬ñÌØ<J
ü8<Í5Ñ�Cïƒ<ßÔó ¯ƒß,2ìü}ô@¸3Ë#¿Á C3À#óëàGï3ü×ïúçÃ­òß<rÄ#ñAó7à�‡óSð�‡??Ü4ÏÇÅA3qÿclô±Ö×î�V¢	®—˜£¾©ÎbsŽ¥�¨›cš:ü˜:¶«¾ú&Vø(âî¦J ü¢Ð× È"7ˆ¨áÍ÷‚€6�}ø´ï«Àz³«ÿ¿š=Ãí³Ï�£óŽ@;(áÖ¼‚(°8rú½ƒŽÏˆ£ß�®3ËëŽrþ°þÖôÁúúüÃã�ãºó.³ë?´q,¿¨>ÿ>¬NÂ~¥Xzu8‘ºOz©TU �@å­ï×uŽª:'€»¸)Fá>;¸9yxéOµ•:>„Žµ$ˆ�ËŠ"à—‘ûGú¤´ˆnäÒI+bÛ¦šÒjeèÕ…›DHD¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¦Ø¸;“›óþ^óöüÿ—¼ý+ÏÚï?Ø8yjªš˜Ñóì>á9¯E$„ê¨ùÉýk?™!,šs3·ƒË*�rÈx_àÊ^–Ï£MÛÿ«=82Y1D?7ÆÏëõÒŒÓá`Å€s?‰0Í¥3‚
s4È|çz=3ù3ó¾¬êëúú»=ÊÏ¯òÔîVÀþ¤
ñ©#©Ã~ÇýUz@†
Ü’Û?†G
«?¨r�/rº–Hè¯W�uÞ×ù»šê†¦P£Æ¬>h)Ua8j]U©s©uY¥Î�jBê°b3ÿšŠš«•�­Y‰¼í„u—øec­ešPQ&k]«È³úkúª0Ú£ëâª;Es‚%QŽm(ÜH
vÅàn×L%èDªÏñEÊ¯Xa7ªT®9ˆ3	¦üšð5^ÂÏ6wší‚„žßKtU6éÁƒaÒQ˜Ò8ö_a!—xÃ×§®q°AñA9|ê¨Ýv b^Ï¡²€_áaOgÕÇ!ßq�‰5ƒ]rŒÕæ—ƒžžäëð×ZY0eJ4zã;ëÃƒ‚¦Ž[�<!2ìÂm£MàF	OïÉ²Jnw-y�…ÅdtláûxiM —‹GÔ©í™ž¤hjUëj¥©T�…ê¡–í‡Õoér«+Þ-òEQµAºèFúª¸Ì–ãŽ�]U_|çÓ**<Q9õîªñB^´Ñ3F}Yâºä‰<’�÷Þµ¸“|ùÙû;Ž[âŸH8ãÒe,‹§À {QÆJ|C4ÂÀáp ¬�Ý?N�1qùß„gçL„æS=Î¿T0t&ð”Ã@°]LÜ@N½ Ï‚n¦KâÀ«=û‡¡2Æª!ŽË@ù�UÏMsªœ+àú£ƒêŽÝŠêøÊoøkˆ|š|ú„„áŸ;<ÓÅÇÄÍ1-!1   !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ,?<?xml version="1.0"?><Features recycledIDs="1" nextValidID="29"><Feature recentID="0" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2818-2864" color="#ccffcc" type="standard"/></Feature><Feature recentID="28" name="3xFLAG" directionality="1" translationMW="2730.72" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2939" maxFusedRunOn="2939" detectionMode="exactProteinMatch"><Segment range="2871-2936" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;three tandem FLAGÂ® epitope tags, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="27" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4464" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="4081" maxFusedRunOn="4081" detectionMode="exactProteinMatch"><Segment range="4465-4476" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="26" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5319-5335" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="M13 rev" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-227" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="187-203" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5291-5309" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="T3 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="248-266" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T3 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="113-134" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="149-154" color="#ffffff" type="standard"/><Segment range="155-172" color="#ffffff" type="standard"/><Segment name="-10" range="173-179" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5958-6062" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2095-2211" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4974-5095" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="748-873" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5135-5270" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3169-3304" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="521-701" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2538-2741" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="RSV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="294-520" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Rous sarcoma virus enhancer/promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4669-4902" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1366-1599" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2234-2537" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2989-3318" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5477-5932" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7094-7682" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3994-4582" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3328-3927" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="AmpR" directionality="1" translationMW="31558.16" type="CDS" allowSegmentOverlaps="0" cleavageArrows="6131" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="6063-6131" color="#ccffcc" type="standard" translated="1"/><Segment range="6132-6923" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>9d09dad2-79cd-4df1-af32-0dbde0e69422</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:9:52">2018.9.13</Created>
<LastModified UTC="8:20:59">2024.7.3</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  g<StrandColors><TopStrand><ColorRange range="2817..2863" colors="black"/><ColorRange range="2864..2869" colors="blue"/><ColorRange range="2936..2938" colors="blue"/></TopStrand><BottomStrand><ColorRange range="2817..2863" colors="black"/><ColorRange range="2864..2869" colors="blue"/><ColorRange range="2936..2938" colors="blue"/></BottomStrand></StrandColors>  8�     Î  î         îÁDÏÖsWÏ»üÌ ñ­¥�87
¿L!3\Ö–!);{iÿC©Þ¦:ðì˜¢ Z!0eÖ­Ýtæa§=LÍi·•uS�8ÔébK¦5žè’è×`—JMv>�AU7ÈØñ �IB	ÆÈúitp.� q}áhóhÑ¢jR>NÆvWîà¤}„øîàåäýã¥>ÖTèú:Ô’"?.(ˆ{‚^Doíê’Ò U»“H3ñt¡S_D»±Í°èáLÍÃQ@0Ç‚sÕZ¡äÁED`#2ÒiL E¤lÆUk
Pô TUY•¥]™&S@NEÆ³J[àø¤Wm8‘ÕžÓ�}¿­ÑEéi™gI5þæˆ�µA‘TwÔêGQ‘UÕIGß7ML<áºÑøA„ZR™•™RIU¡}¤MýõQH#DEUH�N¢¾€®€M4Þ`3Lµ‡8p�GZh¸&HÓMZŽ9¡×M¸£^Ý. ÛSNô5ø8�QŸ•×Nø¨ŽàUí¨ßxâ>�c’�%•»�tj·qøÑ¥Ö�Ôég{éhQlÕ}5E…ÔZh‘•€Ô¨�h*]—Vƒˆ&eZke†I:	
”Y*£‰êæHY�Ð­ö›*ÑýûõÿñÐüPYQBDGDtokH\±Âl)z¥QT  	Ò4Qd‘Ùd×GgW•�UÕ§U­V©±”è¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQßäNL©^”è'ÄìÞKÒ �k�½7H0ó±.DûyÕaÂeFØvÇ�A‡u$Ào’—2ÇÒâˆ‹J@ˆ/ô;ý44RcTØÛµ¤´×Ðˆ/ô;ý44RcTØÛµ¤´×Ù/Ïù{ÏÛóþ^óô¯?k¼ÿ`áåªªjcD<CÏ°û„æ½’Cª Cä:£UNŸañ'S÷Ï³ßÌqËýÉÍþÏ<ÊÏÏs=ß,oÏvÄÀ·ÄãQ{¯·3,Çl5€³3Ì Âà}ÏL±L Â+<FÃó6„Â¿3Mt`PûàÏ7ÃõüÈ+à÷Ë»?}P.LòÈïðHÃÄÌðüúøûÌÿ5ûþ¹ÄÃ°ë|·Ï±üP|Íø#aüÔü#áÏÏ÷Í3ññPLÜØÛ=,uµû�§U¨…F‡‚k¥æ(ïªs˜�œã©cê&ØæŽ¿&­ªï¾‰•¾
8»©„ˆ?(´5È2�â*8s_ýà £ß~-;êð¬êÿïæ�pÁ{lóãèüã�ÐÃŠxu¯ Š,N¾¯`ã³â(÷àkŒÀrúãœ¿¬?µ½0~¾¿0øãÃ¸Ãî¼Ë¬úÏíKïêÏ¿Ï�k°Ÿ©GÖ�CN$n“ÞªU@àP9k{õÅFãªŽ‰à.î
GÑ¸O€Nî^@_Þ:SíeN�¡#­I"#òâˆ‡¸%äFþÑþ©-"[¹4’JØ¶é¦´š™z5EafÑè©£N™`§VTÖM·V­Õ•6ª­ãk’ˆ*/W¶Ö&é¶.äæüÿ—¼ý¿?åï?Jóö»ÏöZª¦¦4CÄ<û¸NkÑI!:ª>@rZÏæHK&œÌíàòÊ§\²Wø2Ÿ×¥³èÓvÿêÏN–LQÍñ³úýt£4øX1`ÏâL3iLÃ ‚œÍ29Þ�LþLüï«:ºþ¾®Ïr³Ã«üµ;•°¿©¼jHêpß±ÿU^†�!‚·$€öÏá‘ÂªÏê KÜ®¥’º+Õà]wµþnÂæº¡©”¨ñ«š
UXN—Uj\Åª]Vis§Z�ƒz¬XŒÁæ¢¦ªågkVbo;aeþXëYf…TI„š×jò,þšþªŒ6¨úøªŽßÑ\à…ITc…J7�±xµÓ	Fú*³üQr«ÖMê•+ŽbÂi¿&‡<D×°³Í�æ»E‡ ¡'·ÒÝMºp`Øt”E¦4Ž=—ØHeÞ0õéë‡\l|PN_:ª7]€Ø—³èl Á8XSÙõqÄÈwÜcâM`×\£5y¥àÅç§¹:ü5Ö–G��R�Þ¸ÎÃúðàà©£†VàOL€»0›hÓxÑ‚Sûòl’›�Ç KC^`aqY8FB>ÞSh%âÑõ*{fg©•zÚ©jUä!Fú¨e»aõ[ú\„ªÊ÷„ËGü‘TmPnº¾ª®3%¸ã£WUWß9ôÂ
Š�ÔN}{ª¼P—ƒ­4LÑŸVx®¹"O$¤=÷­Eî$ß>v~Îã–ø§Ò8ôƒK"éð(Ôq’ŸPÍ0°8\ +#wOÓ L\~wÅáùÓ!9„TÏs¯Å	¼%0Ð,A7¯@sà›©’ø°*Ï~áèL±ªˆc²Ð Á>`UsÓ\Ãê§
ø>¨àú£·bº¾2›þâ_&Ÿ>¡!8gÎÏ4ñqñ3LKHLA  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ,?<?xml version="1.0"?><Features recycledIDs="1" nextValidID="29"><Feature recentID="0" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2818-2925" color="#ccffcc" type="standard"/></Feature><Feature recentID="28" name="3xFLAG" directionality="1" translationMW="2730.72" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3000" maxFusedRunOn="3000" detectionMode="exactProteinMatch"><Segment range="2932-2997" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;three tandem FLAGÂ® epitope tags, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="27" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4525" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="4142" maxFusedRunOn="4142" detectionMode="exactProteinMatch"><Segment range="4526-4537" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="26" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5380-5396" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="M13 rev" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-227" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="187-203" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5352-5370" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="T3 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="248-266" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T3 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="113-134" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="149-154" color="#ffffff" type="standard"/><Segment range="155-172" color="#ffffff" type="standard"/><Segment name="-10" range="173-179" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6019-6123" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2095-2211" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5035-5156" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="748-873" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5196-5331" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3230-3365" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="521-701" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2538-2741" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="RSV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="294-520" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Rous sarcoma virus enhancer/promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4730-4963" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1366-1599" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2234-2537" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3050-3379" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5538-5993" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7155-7743" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4055-4643" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3389-3988" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="AmpR" directionality="1" translationMW="31558.16" type="CDS" allowSegmentOverlaps="0" cleavageArrows="6192" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="6124-6192" color="#ccffcc" type="standard" translated="1"/><Segment range="6193-6984" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>9d09dad2-79cd-4df1-af32-0dbde0e69422</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:9:52">2018.9.13</Created>
<LastModified UTC="8:20:52">2024.7.3</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  g<StrandColors><TopStrand><ColorRange range="2817..2924" colors="black"/><ColorRange range="2925..2930" colors="blue"/><ColorRange range="2997..2999" colors="blue"/></TopStrand><BottomStrand><ColorRange range="2817..2924" colors="black"/><ColorRange range="2925..2930" colors="blue"/><ColorRange range="2997..2999" colors="blue"/></BottomStrand></StrandColors>  =     Þ  #        #ÁDÏÖsWÏ»üÌ ñ­¥�87
¿L!3\Ö–!);{iÿC©Þ¦:ðì˜¢ Z!0eÖ­Ýtæa§=LÍi·•uS�8ÔébK¦5žè’è×`—JMv>�AU7ÈØñ �IB	ÆÈúitp.� q}áhóhÑ¢jR>NÆvWîà¤}„øîàåäýã¥>ÖTèú:Ô’"?.(ˆ{‚^Doíê’Ò U»“H3ñt¡S_D»±Í°èáLÍÃQ@0Ç‚sÕZ¡äÁED`#2ÒiL E¤lÆUk
Pô TUY•¥]™&S@NEÆ³J[àø¤Wm8‘ÕžÓ�}¿­ÑEéi™gI5þæˆ�µA‘TwÔêGQ‘UÕIGß7ML<áºÑøA„ZR™•™RIU¡}¤MýõQH#DEUH�N¢¾€®€M4Þ`3Lµ‡8p�GZh¸&HÓMZŽ9¡×M¸£^Ý. ÛSNô5ø8�QŸ•×Nø¨ŽàUí¨ßxâ>�c’�%•»�tj·qøÑ¥Ö�Ôég{éhQlÕ}5E…ÔZh‘•€Ô¨�h*]—Vƒˆ&eZke†I:	
”Y*£‰êæHY�Ð­ö›*ÑýûõÿñÐüPYQBDGDtokH\±Âl)z¥QT  	Ò4Qd‘Ùd×GgW•�UÕ§U­V©±”è¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQßäO}€ïl3MM�jŠ¡‰­A;T½ö=an¹ãý£ÛbÓ¹=‚gà#U7ÐwFVP¢³¨ezôÁ&…;HƒvâW+{­éÛí}[}_V©¬­>�I¦8QŸÖøôYèÒ"‰‘:ùµêÕÓ�SÑ¶Ö¾EKGV¦ÑX¾´õ;Èí^5‚¸)cÚ.µAâ6¥wu5�«D‡„ÔTÕø�b}
š‘ ÛX:áaFø¯j= a6(#Š>Rí¡4èD™mz…W‡…�ô®X¦T‰éû6`AU�~Ý¸ºX9€Úxr*NëO[‹ƒnûM>fíN/cMØÑ[XSCDB	ÓD88ÔË¸Qj@áöö¾ë‰µ7l××<®ë¨4b*ÿ»yÕaÂeFØvÇ�A‡u$Ào’—2ÇÒâˆ‹J@ˆ/ô;ý44RcTØÛµ¤´×Ðˆ/ô;ý44RcTØÛµ¤´×Ù/Ïù{ÏÛóþ^óô¯?k¼ÿ`áåªªjcD<CÏ°û„æ½’Cª Cä:£UNŸañ'S÷Ï³ßÌqËýÉÍþÏ<ÊÏÏs=ß,oÏvÄÀ·ÄãQ{¯·3,Çl5€³3Ì Âà}ÏL±L Â+<FÃó6„Â¿3Mt`PûàÏ7ÃõüÈ+à÷Ë»?}P.LòÈïðHÃÄÌðüúøûÌÿ5ûþ¹ÄÃ°ë|·Ï±üP|Íø#aüÔü#áÏÏ÷Í3ññPLÜØÛ=,uµû�§U¨…F‡‚k¥æ(ïªs˜�œã©cê&ØæŽ¿&­ªï¾‰•¾
8»©„ˆ?(´5È2�â*8s_ýà £ß~-;êð¬êÿïæ�pÁ{lóãèüã�ÐÃŠxu¯ Š,N¾¯`ã³â(÷àkŒÀrúãœ¿¬?µ½0~¾¿0øãÃ¸Ãî¼Ë¬úÏíKïêÏ¿Ï�k°Ÿ©GÖ�CN$n“ÞªU@àP9k{õÅFãªŽ‰à.î
GÑ¸O€Nî^@_Þ:SíeN�¡#­I"#òâˆ‡¸%äFþÑþ©-"[¹4’JØ¶é¦´š™z5EafÑè©£N™`§VTÖM·V­Õ•6ª­ãk’ˆ*/W¶Ö&é¶.äæüÿ—¼ý¿?åï?Jóö»ÏöZª¦¦4CÄ<û¸NkÑI!:ª>@rZÏæHK&œÌíàòÊ§\²Wø2Ÿ×¥³èÓvÿêÏN–LQÍñ³úýt£4øX1`ÏâL3iLÃ ‚œÍ29Þ�LþLüï«:ºþ¾®Ïr³Ã«üµ;•°¿©¼jHêpß±ÿU^†�!‚·$€öÏá‘ÂªÏê KÜ®¥’º+Õà]wµþnÂæº¡©”¨ñ«š
UXN—Uj\Åª]Vis§Z�ƒz¬XŒÁæ¢¦ªågkVbo;aeþXëYf…TI„š×jò,þšþªŒ6¨úøªŽßÑ\à…ITc…J7�±xµÓ	Fú*³üQr«ÖMê•+ŽbÂi¿&‡<D×°³Í�æ»E‡ ¡'·ÒÝMºp`Øt”E¦4Ž=—ØHeÞ0õéë‡\l|PN_:ª7]€Ø—³èl Á8XSÙõqÄÈwÜcâM`×\£5y¥àÅç§¹:ü5Ö–G��R�Þ¸ÎÃúðàà©£†VàOL€»0›hÓxÑ‚Sûòl’›�Ç KC^`aqY8FB>ÞSh%âÑõ*{fg©•zÚ©jUä!Fú¨e»aõ[ú\„ªÊ÷„ËGü‘TmPnº¾ª®3%¸ã£WUWß9ôÂ
Š�ÔN}{ª¼P—ƒ­4LÑŸVx®¹"O$¤=÷­Eî$ß>v~Îã–ø§Ò8ôƒK"éð(Ôq’ŸPÍ0°8\ +#wOÓ L\~wÅáùÓ!9„TÏs¯Å	¼%0Ð,A7¯@sà›©’ø°*Ï~áèL±ªˆc²Ð Á>`UsÓ\Ãê§
ø>¨àú£·bº¾2›þâ_&Ÿ>¡!8gÎÏ4ñqñ3LKHLA    Œ  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  /»<?xml version="1.0"?><Features nextValidID="29"><Feature recentID="28" name="3xFLAG" directionality="1" translationMW="2730.72" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4056" maxFusedRunOn="4056" detectionMode="exactProteinMatch"><Segment range="3988-4053" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;three tandem FLAGÂ® epitope tags, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="27" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5581" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="5198" maxFusedRunOn="5198" detectionMode="exactProteinMatch"><Segment range="5582-5593" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="26" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6436-6452" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="M13 rev" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-227" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="187-203" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6408-6426" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="T3 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="248-266" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T3 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="113-134" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="149-154" color="#ffffff" type="standard"/><Segment range="155-172" color="#ffffff" type="standard"/><Segment name="-10" range="173-179" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7075-7179" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2095-2211" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6091-6212" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="748-873" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6252-6387" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4286-4421" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="521-701" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2538-2741" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="RSV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="294-520" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Rous sarcoma virus enhancer/promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5786-6019" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1366-1599" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2234-2537" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4106-4435" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6594-7049" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8211-8799" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5111-5699" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4445-5044" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="AmpR" directionality="1" translationMW="31558.16" type="CDS" allowSegmentOverlaps="0" cleavageArrows="7248" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="7180-7248" color="#ccffcc" type="standard" translated="1"/><Segment range="7249-8040" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="ovalbumin" directionality="1" translationMW="42882.53" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4056" maxFusedRunOn="4056"><Segment range="2824-3981" color="#993366" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;chicken ovalbumin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2818-2827" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>9d09dad2-79cd-4df1-af32-0dbde0e69422</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:9:52">2018.9.13</Created>
<LastModified UTC="8:20:42">2024.7.3</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  g<StrandColors><TopStrand><ColorRange range="2817..2830" colors="green"/><ColorRange range="3981..3986" colors="blue"/><ColorRange range="4053..4055" colors="blue"/></TopStrand><BottomStrand><ColorRange range="2817..2830" colors="green"/><ColorRange range="3981..3986" colors="blue"/><ColorRange range="4053..4055" colors="blue"/></BottomStrand></StrandColors>  =Ì     	ù  '|        '|ÁDÏÖsWÏ»üÌ ñ­¥�87
¿L!3\Ö–!);{iÿC©Þ¦:ðì˜¢ Z!0eÖ­Ýtæa§=LÍi·•uS�8ÔébK¦5žè’è×`—JMv>�AU7ÈØñ �IB	ÆÈúitp.� q}áhóhÑ¢jR>NÆvWîà¤}„øîàåäýã¥>ÖTèú:Ô’"?.(ˆ{‚^Doíê’Ò U»“H3ñt¡S_D»±Í°èáLÍÃQ@0Ç‚sÕZ¡äÁED`#2ÒiL E¤lÆUk
Pô TUY•¥]™&S@NEÆ³J[àø¤Wm8‘ÕžÓ�}¿­ÑEéi™gI5þæˆ�µA‘TwÔêGQ‘UÕIGß7ML<áºÑøA„ZR™•™RIU¡}¤MýõQH#DEUH�N¢¾€®€M4Þ`3Lµ‡8p�GZh¸&HÓMZŽ9¡×M¸£^Ý. ÛSNô5ø8�QŸ•×Nø¨ŽàUí¨ßxâ>�c’�%•»�tj·qøÑ¥Ö�Ôég{éhQlÕ}5E…ÔZh‘•€Ô¨�h*]—Vƒˆ&eZke†I:	
”Y*£‰êæHY�Ð­ö›*ÑýûõÿñÐüPYQBDGDtokH\±Âl)z¥QT  	Ò4Qd‘Ùd×GgW•�UÕ§U­V©±”è¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQßäO}‚øsŽë¼ùÕâ<ë­ö-Ü<ñ°áÒ?Á°H ÏOØ 8ÀÂÌ³‹?€Þ�C?�2³�„ãMâñ8WCS0Ì 3ìñ¯N	Sàã� <Ä ýBæ1Žg.>Ç£Š0ã­ðND"4<ÌèàMàƒ„_ó ÌÛ�Èn…ø1A3âàÜS ÀÛ MØ­MìG#Ó0ðCìsâx#Ï7GðÎ8ÏxÎèÎ^38Ü ôp�0�‚S3Ïëó³«½AŽ0ãsãàÂ"ƒŸ]A1kƒvƒ:,O; Û8ðó¬üãà��HwSÐ,�ôqøÑ>âŽÆ²Ì¨í#dkŸˆÍãÛQ>tGÔ"´Çl3›Ñ>Ï‚®žç÷,3ƒÔÌÎ3æ€Ä�PÍ¾?ŒƒÓ�AN½ƒ�4l:ïÒxÄ^¬ÓBF~:åÝQ{ÑÑù…mM·óQ
ê@NÝµ¬ôç9èÇÍ5ù0x8>û:óÒwæ‚ÓŒ.Îþ8ß2-M;àAâ¼ø�ós6û¸ÓÑz�ÑàøE:GÔ»Üa�ÿ!”Ò Q=þß½‹º¾ƒ½Žv¿Î?‰àCS´€ÿF.¦ÒŸ´ôàôûáB7ãN·´Ë­±ÈN<5ku:oTËdÃ³Z¢ø#‹Ž ÎR64aãPxÝA!ã©N Ô]¢?m¡ƒ¨H>íãácC‰íú×µ`¿a'ÐCßlEo:ùÐ<ÿ»Aï¸ðlô¨œõ€¸û±�Qö!¦Tm‡lyÔq‡RLðy)s,} ±þ Xˆ´¤�RÿC¿ÓCE&5M�»ZKM}�RÿC¿ÓCE&5M�»ZKM}’üÿ—¼ý¿?åï?Jóö»ÏöZª¦¦4CÄ<û¸NkÑI!:ª>Cª5Téöu?pŒû=üÇ¿Ð<œßìóÌ¬ñçü÷3ÝòÆð|÷lL|N5ºûs2ÀìvÃPˆ0qÃ<Â.ÜðDËÀ"³Äl?3`ñ(L+ðàó4×F¾ó|?POÌ‚¾|°Ë³ñ÷Ð5à4Ï,Žÿ�<LÏ �Ï¯�¼Ïó_¿ëœL;·Ë|ñË�ÅÌß‚6ÍOÂ>üÿqüÓ?ÍÇý�³ÒÇ[_¸uZˆThx&º^bŽú§9ˆÎ:–>¢mŽhëò`êÚ®ûè™[à£‹º˜A(ƒò‹C\ƒ ˆÞ"£‡5ÿÞ
 Ú=÷âÓ¾¯êÎ¯þþh÷¶Ï>>�Î8 ì8 7‡Zò¢ÀáËêö;>"�~¸Ì/®9ËúÃû[ÓëëóŽ<;Œ>ëÌºÏ¬þÑÄ°nþ <ûüø±;	ú”}aéÔ4âFé=ê¥QT –·¿\QÔn:¨èžîà¤}„øîàåäýã¥>ÖTèú:Ô’"?.(ˆ{‚^Doíê’Ò U»“I$­‹nškI©—£TVm!^Šš4é–
ueMdÛujÝYSjªÞ6¹(‚¢õ{mbn›bàîNoÏù{ÏÛóþ^óô¯?k¼ÿ`áåªªjcD<CÏ°û„æ½’Cª Cä'õ¬þd„²iÌÎÞ,ªuË!àƒ)ýz[>�7oþ¬ôàÉdÅüß?¯×J3O…ƒÌþ$Ã6”Ì0")ÌÓ!ó�èôÏäÏÎú³«¯ëêì÷+<:¿ËS¹[ ;ú�+Æ¤Ž§ûõUèi+rHlþ*¬þ¡Ê½ÊêY {¢½^×{_æì.kª™@j�°ù ¥U„á©uV¥ÌZ¥Õf—:u©7ªÀˆÌþj*j®Vvµf&ó¶Ö_á•Žµ–hUAD˜I­v¯"Ïé¯ê¨Ãj�¯Š¨íýÎT•F8Q´ #q )Û�»]0”o¡«?Å*½a„Þ©R¸æ Ì&›òhsÀÔM{<ÙÞk´Xz
{}-ÑTÛ§‡IDZcHãÙ}„†]ã^ž¸uÆÁÅåóª£uØ m‰{>†Ê|……=ŸWL‡}Æ>$ÖuÊ3Wš^^z{“¯Ã]idxÁ•(Ð}ëŒì?¯
š8enð„È³	¶�7€=%?¿&É)¹Üp´5æ‘Ñ³„d#íá¥6‚^-R§¶fz‘¡©W­ª–¥PnBoª†[¶U¿¥ÈJ¬¯xL´ÉFÕë¡êªã2[Ž:5uU}óŸL ¨¨ðmDç×º«Å	x:ÓDÍõgŠë’$òJCßzÔ^âMóçgìî9oŠ} ã�H1”².Ÿ�íG)ðeÓ…À²7tý:ÅÇç|^Ÿ�2˜EL÷:üPPÁÐ›ÂSÁt3q:ô W>	º™/‹¬÷î„Ë¨†;- æW=5Ì>ªp¯ƒêŽª;v+«ã)¿á® eòióê†|ìóO4Ä´„Ä    ú  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  .ù<?xml version="1.0"?><Features nextValidID="29"><Feature recentID="28" name="AmpR" directionality="1" translationMW="31558.16" type="CDS" allowSegmentOverlaps="0" cleavageArrows="8382" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="8314-8382" color="#ccffcc" type="standard" translated="1"/><Segment range="8383-9174" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="27" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="5579-6178" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6245-6833" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9345-9933" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7728-8183" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5240-5569" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2234-2537" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1366-1599" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6920-7153" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="RSV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="294-520" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Rous sarcoma virus enhancer/promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2538-2741" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="521-701" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5420-5555" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7386-7521" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="748-873" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7225-7346" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2095-2211" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8209-8313" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="149-154" color="#ffffff" type="standard"/><Segment range="155-172" color="#ffffff" type="standard"/><Segment name="-10" range="173-179" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="113-134" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="T3 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="248-266" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T3 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7542-7560" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="187-203" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="M13 rev" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-227" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7570-7586" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6715" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6332" maxFusedRunOn="6332" detectionMode="exactProteinMatch"><Segment range="6716-6727" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="2" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2818-2827" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="1" name="Sarm1-m" directionality="1" translationMW="84008.92" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="5190" maxFusedRunOn="5190"><Segment range="2824-5115" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="3xFLAG" directionality="1" translationMW="2730.72" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="5190" maxFusedRunOn="5190" detectionMode="exactProteinMatch"><Segment range="5122-5187" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;three tandem FLAGÂ® epitope tags, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>9d09dad2-79cd-4df1-af32-0dbde0e69422</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:9:52">2018.9.13</Created>
<LastModified UTC="8:15:53">2024.7.3</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Á<StrandColors><TopStrand><ColorRange range="2817..2822" colors="blue"/><ColorRange range="4453..4572" colors="blue"/><ColorRange range="5115..5120" colors="blue"/><ColorRange range="5187..5189" colors="blue"/></TopStrand><BottomStrand><ColorRange range="2817..2822" colors="blue"/><ColorRange range="4453..4572" colors="blue"/><ColorRange range="5115..5120" colors="blue"/><ColorRange range="5187..5189" colors="blue"/></BottomStrand></StrandColors>  <¶     	ø  'v        'vÁDÏÖsWÏ»üÌ ñ­¥�87
¿L!3\Ö–!);{iÿC©Þ¦:ðì˜¢ Z!0eÖ­Ýtæa§=LÍi·•uS�8ÔébK¦5žè’è×`—JMv>�AU7ÈØñ �IB	ÆÈúitp.� q}áhóhÑ¢jR>NÆvWîà¤}„øîàåäýã¥>ÖTèú:Ô’"?.(ˆ{‚^Doíê’Ò U»“H3ñt¡S_D»±Í°èáLÍÃQ@0Ç‚sÕZ¡äÁED`#2ÒiL E¤lÆUk
Pô TUY•¥]™&S@NEÆ³J[àø¤Wm8‘ÕžÓ�}¿­ÑEéi™gI5þæˆ�µA‘TwÔêGQ‘UÕIGß7ML<áºÑøA„ZR™•™RIU¡}¤MýõQH#DEUH�N¢¾€®€M4Þ`3Lµ‡8p�GZh¸&HÓMZŽ9¡×M¸£^Ý. ÛSNô5ø8�QŸ•×Nø¨ŽàUí¨ßxâ>�c’�%•»�tj·qøÑ¥Ö�Ôég{éhQlÕ}5E…ÔZh‘•€Ô¨�h*]—Vƒˆ&eZke†I:	
”Y*£‰êæHY�Ð­ö›*ÑýûõÿñÐüPYQBDGDtokH\±Âl)z¥QT  	Ò4Qd‘Ùd×GgW•�UÕ§U­V©±”è¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQßäO}‚øsŽë¼ùÕâ<ë­ö-Ü<ñ°áÒ?Á°H ÏOØ 8ÀÂÌ³‹?€Þ�C?�2³�„ãMâñ8WCS0Ì 3ìñ¯N	Sàã� <Ä ýBæ1Žg.>Ç£Š0ã­ðND"4<ÌèàMàƒ„_ó ÌÛ�Èn…ø1A3âàÜS ÀÛ MØ­MìG#Ó0ðCìsâx#Ï7GðÎ8ÏxÎèÎ^38Ü ôp�0�‚S3Ïëó³«½AŽ0ãsãàÂ"ƒŸ]A1kƒvƒ:,O; Û8ðó¬üãà��HwSÐ,�ôqøÑ>âŽÆ²Ì¨í#dkŸˆÍãÛQ>tGÔ"´Çl3›Ñ>Ï‚®žç÷,3ƒÔÌÎ3æ€Ä�PÍ¾?ŒƒÓ�AN½ƒ�4l:ïÒxÄ^¬ÓBF~:åÝQ{ÑÑù…mM·óQ
ê@NÝµ¬ôç9èÇÍ5ù0x8>û:óÒwæ‚ÓŒ.Îþ8ß2-M;àAâ¼ø�ós6û¸ÓÑz�ÑàøE:GÔ»Üa�ÿ!”Ò Q=þß½‹º¾ƒ½Žv¿Î?‰àCS´€ÿF.¦ÒŸ´ôàôûáB7ãN·´Ë­±ÈN<5ku:oTËdÃ³Z¢ø#‹Ž ÎR64aãPxÝA!ã©N Ô]¢?m¡ƒ¨H>íãácC‰íú×µ`¿a'ÐCßlEo:ùÐ<ÿ»Aï¸ðlô¨œõ€¸øyÕaÂeFØvÇ�A‡u$Ào’—2ÇÒâˆ‹J@ˆ/ô;ý44RcTØÛµ¤´×Ðˆ/ô;ý44RcTØÛµ¤´×Ù/Ïù{ÏÛóþ^óô¯?k¼ÿ`áåªªjcD<CÏ°û„æ½’Cª Cä:£UNŸañ'S÷Ï³ßÌqËýÉÍþÏ<ÊÏÏs=ß,oÏvÄÀ·ÄãQ{¯·3,Çl5€³3Ì Âà}ÏL±L Â+<FÃó6„Â¿3Mt`PûàÏ7ÃõüÈ+à÷Ë»?}P.LòÈïðHÃÄÌðüúøûÌÿ5ûþ¹ÄÃ°ë|·Ï±üP|Íø#aüÔü#áÏÏ÷Í3ññPLÜØÛ=,uµû�§U¨…F‡‚k¥æ(ïªs˜�œã©cê&ØæŽ¿&­ªï¾‰•¾
8»©„ˆ?(´5È2�â*8s_ýà £ß~-;êð¬êÿïæ�pÁ{lóãèüã�ÐÃŠxu¯ Š,N¾¯`ã³â(÷àkŒÀrúãœ¿¬?µ½0~¾¿0øãÃ¸Ãî¼Ë¬úÏíKïêÏ¿Ï�k°Ÿ©GÖ�CN$n“ÞªU@àP9k{õÅFãªŽ‰à.î
GÑ¸O€Nî^@_Þ:SíeN�¡#­I"#òâˆ‡¸%äFþÑþ©-"[¹4’JØ¶é¦´š™z5EafÑè©£N™`§VTÖM·V­Õ•6ª­ãk’ˆ*/W¶Ö&é¶.äæüÿ—¼ý¿?åï?Jóö»ÏöZª¦¦4CÄ<û¸NkÑI!:ª>@rZÏæHK&œÌíàòÊ§\²Wø2Ÿ×¥³èÓvÿêÏN–LQÍñ³úýt£4øX1`ÏâL3iLÃ ‚œÍ29Þ�LþLüï«:ºþ¾®Ïr³Ã«üµ;•°¿©¼jHêpß±ÿU^†�!‚·$€öÏá‘ÂªÏê KÜ®¥’º+Õà]wµþnÂæº¡©”¨ñ«š
UXN—Uj\Åª]Vis§Z�ƒz¬XŒÁæ¢¦ªågkVbo;aeþXëYf…TI„š×jò,þšþªŒ6¨úøªŽßÑ\à…ITc…J7�±xµÓ	Fú*³üQr«ÖMê•+ŽbÂi¿&‡<D×°³Í�æ»E‡ ¡'·ÒÝMºp`Øt”E¦4Ž=—ØHeÞ0õéë‡\l|PN_:ª7]€Ø—³èl Á8XSÙõqÄÈwÜcâM`×\£5y¥àÅç§¹:ü5Ö–G��R�Þ¸ÎÃúðàà©£†VàOL€»0›hÓxÑ‚Sûòl’›�Ç KC^`aqY8FB>ÞSh%âÑõ*{fg©•zÚ©jUä!Fú¨e»aõ[ú\„ªÊ÷„ËGü‘TmPnº¾ª®3%¸ã£WUWß9ôÂ
Š�ÔN}{ª¼P—ƒ­4LÑŸVx®¹"O$¤=÷­Eî$ß>v~Îã–ø§Ò8ôƒK"éð(Ôq’ŸPÍ0°8\ +#wOÓ L\~wÅáùÓ!9„TÏs¯Å	¼%0Ð,A7¯@sà›©’ø°*Ï~áèL±ªˆc²Ð Á>`UsÓ\Ãê§
ø>¨àú£·bº¾2›þâ_&Ÿ>¡!8gÎÏ4ñqñ3LKHLA    ú  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  .ù<?xml version="1.0"?><Features nextValidID="29"><Feature recentID="28" name="3xFLAG" directionality="1" translationMW="2730.72" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="5184" maxFusedRunOn="5184" detectionMode="exactProteinMatch"><Segment range="5116-5181" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;three tandem FLAGÂ® epitope tags, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="27" name="Sarm1-m" directionality="1" translationMW="84008.92" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="5184" maxFusedRunOn="5184"><Segment range="2824-5115" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2818-2827" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="26" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6709" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6326" maxFusedRunOn="6326" detectionMode="exactProteinMatch"><Segment range="6710-6721" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="25" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7564-7580" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="M13 rev" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-227" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="187-203" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7536-7554" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="T3 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="248-266" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T3 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="113-134" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="149-154" color="#ffffff" type="standard"/><Segment range="155-172" color="#ffffff" type="standard"/><Segment name="-10" range="173-179" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8203-8307" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2095-2211" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7219-7340" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="748-873" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7380-7515" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5414-5549" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="521-701" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2538-2741" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="RSV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="294-520" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Rous sarcoma virus enhancer/promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6914-7147" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1366-1599" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2234-2537" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5234-5563" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7722-8177" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9339-9927" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6239-6827" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="5573-6172" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="AmpR" directionality="1" translationMW="31558.16" type="CDS" allowSegmentOverlaps="0" cleavageArrows="8376" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="8308-8376" color="#ccffcc" type="standard" translated="1"/><Segment range="8377-9168" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ><Notes>
<UUID>9d09dad2-79cd-4df1-af32-0dbde0e69422</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:9:52">2018.9.13</Created>
<LastModified UTC="8:6:47">2024.7.3</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   ­<StrandColors><TopStrand><ColorRange range="5181..5183" colors="blue"/></TopStrand><BottomStrand><ColorRange range="5181..5183" colors="blue"/></BottomStrand></StrandColors>  <"     
   'v        'vÁDÏÖsWÏ»üÌ ñ­¥�87
¿L!3\Ö–!);{iÿC©Þ¦:ðì˜¢ Z!0eÖ­Ýtæa§=LÍi·•uS�8ÔébK¦5žè’è×`—JMv>�AU7ÈØñ �IB	ÆÈúitp.� q}áhóhÑ¢jR>NÆvWîà¤}„øîàåäýã¥>ÖTèú:Ô’"?.(ˆ{‚^Doíê’Ò U»“H3ñt¡S_D»±Í°èáLÍÃQ@0Ç‚sÕZ¡äÁED`#2ÒiL E¤lÆUk
Pô TUY•¥]™&S@NEÆ³J[àø¤Wm8‘ÕžÓ�}¿­ÑEéi™gI5þæˆ�µA‘TwÔêGQ‘UÕIGß7ML<áºÑøA„ZR™•™RIU¡}¤MýõQH#DEUH�N¢¾€®€M4Þ`3Lµ‡8p�GZh¸&HÓMZŽ9¡×M¸£^Ý. ÛSNô5ø8�QŸ•×Nø¨ŽàUí¨ßxâ>�c’�%•»�tj·qøÑ¥Ö�Ôég{éhQlÕ}5E…ÔZh‘•€Ô¨�h*]—Vƒˆ&eZke†I:	
”Y*£‰êæHY�Ð­ö›*ÑýûõÿñÐüPYQBDGDtokH\±Âl)z¥QT  	Ò4Qd‘Ùd×GgW•�UÕ§U­V©±”è¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQßäO}‚øsŽë¼ùÕâ<ë­ö-Ü<ñ°áÒ?Á°H ÏOØ 8ÀÂÌ³‹?€Þ�C?�2³�„ãMâñ8WCS0Ì 3ìñ¯N	Sàã� <Ä ýBæ1Žg.>Ç£Š0ã­ðND"4<ÌèàMàƒ„_ó ÌÛ�Èn…ø1A3âàÜS ÀÛ MØ­MìG#Ó0ðCìsâx#Ï7GðÎ8ÏxÎèÎ^38Ü ôp�0�‚S3Ïëó³«½AŽ0ãsãàÂ"ƒŸ]A1kƒvƒ:,O; Û8ðó¬üãà��HwSÐ,�ôqøÑ>âŽÆ²Ì¨í#dkŸˆÍãÛQ>tGÔ"´Çl3›Ñ>Ï‚®žç÷,3ƒÔÌÎ3æ€Ä�PÍ¾?ŒƒÓ�AN½ƒ�4l:ïÒxÄ^¬ÓBF~:åÝQ{ÑÑù…mM·óQ
ê@NÝµ¬ôç9èÇÍ5ù0x8>û:óÒwæ‚ÓŒ.Îþ8ß2-M;àAâ¼ø�ós6û¸ÓÑz�ÑàøE:GÔ»Üa�ÿ!”Ò Q=þß½‹º¾ƒ½Žv¿Î?‰àCS´€ÿF.¦ÒŸ´ôàôûáB7ãN·´Ë­±ÈN<5ku:oTËdÃ³Z¢ø#‹Ž ÎR64aãPxÝA!ã©N Ô]¢?m¡ƒ¨H>íãácC‰íú×µ`¿a'ÐCßlEo:ùÐ<ÿ»Aï¸ðlô¨œõ€¸øyÕaÂeFØvÇ�A‡u!Ào’—2ÇÒâˆ‹J@ˆ/ô;ý44RcTØÛµ¤´×Ðˆ/ô;ý44RcTØÛµ¤´×Ù/Ïù{ÏÛóþ^óô¯?k¼ÿ`áåªªjcD<CÏ°û„æ½’Cª Cä:£UNŸañ'S÷Ï³ßÌqËýÉÍþÏ<ÊÏÏs=ß,oÏvÄÀ·ÄãQ{¯·3,Çl5€³3Ì Âà}ÏL±L Â+<FÃó6„Â¿3Mt`PûàÏ7ÃõüÈ+à÷Ë»?}P.LòÈïðHÃÄÌðüúøûÌÿ5ûþ¹ÄÃ°ë|·Ï±üP|Íø#aüÔü#áÏÏ÷Í3ññPLÜØÛ=,uµû�§U¨…F‡‚k¥æ(ïªs˜�œã©cê&ØæŽ¿&­ªï¾‰•¾
8»©„ˆ?(´5È2�â*8s_ýà £ß~-;êð¬êÿïæ�pÁ{lóãèüã�ÐÃŠxu¯ Š,N¾¯`ã³â(÷àkŒÀrúãœ¿¬?µ½0~¾¿0øãÃ¸Ãî¼Ë¬úÏíKïêÏ¿Ï�k°Ÿ©GÖ�CN$n“ÞªU@àP9k{õÅFãªŽ‰à.î
GÑ¸O€Nî^@_Þ:SíeN�¡#­I"#òâˆ‡¸%äFþÑþ©-"[¹4’JØ¶é¦´š™z5EafÑè©£N™`§VTÖM·V­Õ•6ª­ãk’ˆ*/W¶Ö&é¶.äæüÿ—¼ý¿?åï?Jóö»ÏöZª¦¦4CÄ<û¸NkÑI!:ª>@rZÏæHK&œÌíàòÊ§\²Wø2Ÿ×¥³èÓvÿêÏN–LQÍñ³úýt£4øX1`ÏâL3iLÃ ‚œÍ29Þ�LþLüï«:ºþ¾®Ïr³Ã«üµ;•°¿©¼jHêpß±ÿU^†�!‚·$€öÏá‘ÂªÏê KÜ®¥’º+Õà]wµþnÂæº¡©”¨ñ«š
UXN—Uj\Åª]Vis§Z�ƒz¬XŒÁæ¢¦ªågkVbo;aeþXëYf…TI„š×jò,þšþªŒ6¨úøªŽßÑ\à…ITc…J7�±xµÓ	Fú*³üQr«ÖMê•+ŽbÂi¿&‡<D×°³Í�æ»E‡ ¡'·ÒÝMºp`Øt”E¦4Ž=—ØHeÞ0õéë‡\l|PN_:ª7]€Ø—³èl Á8XSÙõqÄÈwÜcâM`×\£5y¥àÅç§¹:ü5Ö–G��R�Þ¸ÎÃúðàà©£†VàOL€»0›hÓxÑ‚Sûòl’›�Ç KC^`aqY8FB>ÞSh%âÑõ*{fg©•zÚ©jUä!Fú¨e»aõ[ú\„ªÊ÷„ËGü‘TmPnº¾ª®3%¸ã£WUWß9ôÂ
Š�ÔN}{ª¼P—ƒ­4LÑŸVx®¹"O$¤=÷­Eî$ß>v~Îã–ø§Ò8ôƒK"éð(Ôq’ŸPÍ0°8\ +#wOÓ L\~wÅáùÓ!9„TÏs¯Å	¼%0Ð,A7¯@sà›©’ø°*Ï~áèL±ªˆc²Ð Á>`UsÓ\Ãê§
ø>¨àú£·bº¾2›þâ_&Ÿ>¡!8gÎÏ4ñqñ3LKHLA    ú  =  ?  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  /<?xml version="1.0"?><Features nextValidID="29"><Feature recentID="28" name="3xFLAG" directionality="1" translationMW="2730.72" type="CDS" allowSegmentOverlaps="0" cleavageArrows="5181" consecutiveTranslationNumbering="1" maxRunOn="5184" maxFusedRunOn="5184" detectionMode="exactProteinMatch"><Segment range="5116-5181" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;three tandem FLAGÂ® epitope tags, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="27" name="Sarm1-m" directionality="1" translationMW="84008.92" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="5184" maxFusedRunOn="5184"><Segment range="2824-5115" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2818-2827" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="26" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6709" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6326" maxFusedRunOn="6326" detectionMode="exactProteinMatch"><Segment range="6710-6721" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="25" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7564-7580" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="M13 rev" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-227" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="187-203" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7536-7554" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="T3 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="248-266" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T3 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="113-134" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="149-154" color="#ffffff" type="standard"/><Segment range="155-172" color="#ffffff" type="standard"/><Segment name="-10" range="173-179" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8203-8307" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2095-2211" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7219-7340" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="748-873" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7380-7515" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5414-5549" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="521-701" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2538-2741" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="RSV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="294-520" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Rous sarcoma virus enhancer/promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6914-7147" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1366-1599" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2234-2537" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5234-5563" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7722-8177" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9339-9927" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6239-6827" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="5573-6172" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="AmpR" directionality="1" translationMW="31558.16" type="CDS" allowSegmentOverlaps="0" cleavageArrows="8376" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="8308-8376" color="#ccffcc" type="standard" translated="1"/><Segment range="8377-9168" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ><Notes>
<UUID>9d09dad2-79cd-4df1-af32-0dbde0e69422</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:9:52">2018.9.13</Created>
<LastModified UTC="8:6:29">2024.7.3</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  9º     	ç  '1        '1ÁDÏÖsWÏ»üÌ ñ­¥�87
¿L!3\Ö–!);{iÿC©Þ¦:ðì˜¢ Z!0eÖ­Ýtæa§=LÍi·•uS�8ÔébK¦5žè’è×`—JMv>�AU7ÈØñ �IB	ÆÈúitp.� q}áhóhÑ¢jR>NÆvWîà¤}„øîàåäýã¥>ÖTèú:Ô’"?.(ˆ{‚^Doíê’Ò U»“H3ñt¡S_D»±Í°èáLÍÃQ@0Ç‚sÕZ¡äÁED`#2ÒiL E¤lÆUk
Pô TUY•¥]™&S@NEÆ³J[àø¤Wm8‘ÕžÓ�}¿­ÑEéi™gI5þæˆ�µA‘TwÔêGQ‘UÕIGß7ML<áºÑøA„ZR™•™RIU¡}¤MýõQH#DEUH�N¢¾€®€M4Þ`3Lµ‡8p�GZh¸&HÓMZŽ9¡×M¸£^Ý. ÛSNô5ø8�QŸ•×Nø¨ŽàUí¨ßxâ>�c’�%•»�tj·qøÑ¥Ö�Ôég{éhQlÕ}5E…ÔZh‘•€Ô¨�h*]—Vƒˆ&eZke†I:	
”Y*£‰êæHY�Ð­ö›*ÑýûõÿñÐüPYQBDGDtokH\±Âl)z¥QT  	Ò4Qd‘Ùd×GgW•�UÕ§U­V©±”è¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQßäO}‚øsŽë¼ùÕâ<ë­ö-Ü<ñ°áÒ?Á°H ÏOØ 8ÀÂÌ³‹?€Þ�C?�2³�„ãMâñ8WCS0Ì 3ìñ¯N	Sàã� <Ä ýBæ1Žg.>Ç£Š0ã­ðND"4<ÌèàMàƒ„_ó ÌÛ�Èn…ø1A3âàÜS ÀÛ MØ­MìG#Ó0ðCìsâx#Ï7GðÎ8ÏxÎèÎ^38Ü ôp�0�‚S3Ïëó³«½AŽ0ãsãàÂ"ƒŸ]A1kƒvƒ:,O; Û8ðó¬üãà��HwSÐ,�ôqøÑ>âŽÆ²Ì¨í#dkŸˆÍãÛQ>tGÔ"´Çl3›Ñ>Ï‚®žç÷,3ƒÔÌÎ3æ€Ä�PÍ¾?ŒƒÓ�AN½ƒ�4l:ïÒxÄ^¬ÓBF~:åÝQ{ÑÑù…mM·óQ
ê@NÝµ¬ôç9èÇÍ5ù0x8>û:óÒwæ‚ÓŒ.Îþ8ß2-M;àAâ¼ø�ós6û¸ÓÑz�ÑàøE:GÔ»Üa�ÿ!”Ò Q=þß½‹º¾ƒ½Žv¿Î?‰àCS´€ÿF.¦ÒŸ´ôàôûáB7ãN·´Ë­±ÈN<5ku:oTËdÃ³Z¢ø#‹Ž ÎR64aãPxÝA!ã©N Ô]¢?m¡ƒ¨H>íãácC‰íú×µ`¿a'ÐCßlEo:ùÐ<ÿ»Aï¸ðlô¨œõ€¸û¼J\ËH,ˆ"-) T¿ÐïôÐÑI�ScnÖ’Ó_B T¿ÐïôÐÑI�ScnÖ’Ó_d¿?åï?oÏù{ÏÒ¼ý®óýƒ‡–ª©©�ñ>ÃîšôRHAª��ê�U:}‡Ä�OÜ#>Ï1Ç/ô'7û<ó+<yÿ=Ì÷|±¼=Ûß�Eî¾ÜÌ°;°Ô"ÌpÏ0ƒ�÷<2Å0¬ñÌØ<J
ü8<Í5Ñ�Cïƒ<ßÔó ¯ƒß,2ìü}ô@¸3Ë#¿Á C3À#óëàGï3ü×ïúçÃ­òß<rÄ#ñAó7à�‡óSð�‡??Ü4ÏÇÅA3qÿclô±Ö×î�V¢	®—˜£¾©ÎbsŽ¥�¨›cš:ü˜:¶«¾ú&Vø(âî¦J ü¢Ð× È"7ˆ¨áÍ÷‚€6�}ø´ï«Àz³«ÿ¿š=Ãí³Ï�£óŽ@;(áÖ¼‚(°8rú½ƒŽÏˆ£ß�®3ËëŽrþ°þÖôÁúúüÃã�ãºó.³ë?´q,¿¨>ÿ>¬NÂ~¥Xzu8‘ºOz©TU �@å­ï×uŽª:'€»¸)Fá>;¸9yxéOµ•:>„Žµ$ˆ�ËŠ"à—‘ûGú¤´ˆnäÒI+bÛ¦šÒjeèÕ…›DHD¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¦Ø¸;“›óþ^óöüÿ—¼ý+ÏÚï?Ø8yjªš˜Ñóì>á9¯E$„ê¨ùÉýk?™!,šs3·ƒË*�rÈx_àÊ^–Ï£MÛÿ«=82Y1D?7ÆÏëõÒŒÓá`Å€s?‰0Í¥3‚
s4È|çz=3ù3ó¾¬êëúú»=ÊÏ¯òÔîVÀþ¤
ñ©#©Ã~ÇýUz@†
Ü’Û?†G
«?¨r�/rº–Hè¯W�uÞ×ù»šê†¦P£Æ¬>h)Ua8j]U©s©uY¥Î�jBê°b3ÿšŠš«•�­Y‰¼í„u—øec­ešPQ&k]«È³úkúª0Ú£ëâª;Es‚%QŽm(ÜH
vÅàn×L%èDªÏñEÊ¯Xa7ªT®9ˆ3	¦üšð5^ÂÏ6wší‚„žßKtU6éÁƒaÒQ˜Ò8ö_a!—xÃ×§®q°AñA9|ê¨Ýv b^Ï¡²€_áaOgÕÇ!ßq�‰5ƒ]rŒÕæ—ƒžžäëð×ZY0eJ4zã;ëÃƒ‚¦Ž[�<!2ìÂm£MàF	OïÉ²Jnw-y�…ÅdtláûxiM —‹GÔ©í™ž¤hjUëj¥©T�…ê¡–í‡Õoér«+Þ-òEQµAºèFúª¸Ì–ãŽ�]U_|çÓ**<Q9õîªñB^´Ñ3F}Yâºä‰<’�÷Þµ¸“|ùÙû;Ž[âŸH8ãÒe,‹§À {QÆJ|C4ÂÀáp ¬�Ý?N�1qùß„gçL„æS=Î¿T0t&ð”Ã@°]LÜ@N½ Ï‚n¦KâÀ«=û‡¡2Æª!ŽË@ù�UÏMsªœ+àú£ƒêŽÝŠêøÊoøkˆ|š|ú„„áŸ;<ÓÅÇÄÍ1-!1     ú  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ,À<?xml version="1.0"?><Features nextValidID="28"><Feature recentID="27" name="Sarm1-m" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="5130" maxFusedRunOn="5130"><Segment range="2824-5115" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2818-2827" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="26" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6640" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6257" maxFusedRunOn="6257" detectionMode="exactProteinMatch"><Segment range="6641-6652" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="25" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7495-7511" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="M13 rev" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-227" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="187-203" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7467-7485" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="T3 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="248-266" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T3 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="113-134" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="149-154" color="#ffffff" type="standard"/><Segment range="155-172" color="#ffffff" type="standard"/><Segment name="-10" range="173-179" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8134-8238" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2095-2211" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7150-7271" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="748-873" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7311-7446" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5345-5480" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="521-701" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2538-2741" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="RSV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="294-520" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Rous sarcoma virus enhancer/promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6845-7078" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1366-1599" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2234-2537" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5165-5494" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7653-8108" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9270-9858" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6170-6758" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="5504-6103" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="AmpR" directionality="1" translationMW="31558.16" type="CDS" allowSegmentOverlaps="0" cleavageArrows="8307" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="8239-8307" color="#ccffcc" type="standard" translated="1"/><Segment range="8308-9099" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ><Notes>
<UUID>9d09dad2-79cd-4df1-af32-0dbde0e69422</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:9:52">2018.9.13</Created>
<LastModified UTC="8:6:25">2024.7.3</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  7¶      T  !         !ÁDÏÖsWÏ»üÌ ñ­¥�87
¿L!3\Ö–!);{iÿC©Þ¦:ðì˜¢ Z!0eÖ­Ýtæa§=LÍi·•uS�8ÔébK¦5žè’è×`—JMv>�AU7ÈØñ �IB	ÆÈúitp.� q}áhóhÑ¢jR>NÆvWîà¤}„øîàåäýã¥>ÖTèú:Ô’"?.(ˆ{‚^Doíê’Ò U»“H3ñt¡S_D»±Í°èáLÍÃQ@0Ç‚sÕZ¡äÁED`#2ÒiL E¤lÆUk
Pô TUY•¥]™&S@NEÆ³J[àø¤Wm8‘ÕžÓ�}¿­ÑEéi™gI5þæˆ�µA‘TwÔêGQ‘UÕIGß7ML<áºÑøA„ZR™•™RIU¡}¤MýõQH#DEUH�N¢¾€®€M4Þ`3Lµ‡8p�GZh¸&HÓMZŽ9¡×M¸£^Ý. ÛSNô5ø8�QŸ•×Nø¨ŽàUí¨ßxâ>�c’�%•»�tj·qøÑ¥Ö�Ôég{éhQlÕ}5E…ÔZh‘•€Ô¨�h*]—Vƒˆ&eZke†I:	
”Y*£‰êæHY�Ð­ö›*ÑýûõÿñÐüPYQBDGDtokH\±Âl)z¥QT  	Ò4Qd‘Ùd×GgW•�UÕ§U­V©±”è¯2�—§	X?>?\óö‡-e‡&+öIsÖ@z½¡ËXjp•ã÷ ÒvÔ‰¶cÔœÿæ‡-aÂVÏƒi�Òv¦êùè4�¹É¤¶Îiöc
 ÒvÖ dÂ¡íÀ½K½ÿhrÖ**ƒ}V×êõX²]{Ïö‡5`0�Èœ ¹™M²¤…òÑ³àG=½Î*‡ïdQßäX!5N+|ý¾�Ã•ÃÝJÓ"ðÄ@Æ=ùÃS‡øR¶ã}ðÔãò?ƒ÷û!÷ø~pÈÒ:ÓÎÇÝ…4ÜzëRóØüPç/A3}ºëPqÃ^uÌñR±þ_6Äá@Ûµ5ÛàÔàI×�t÷\¹›`ñÔÑEÃmB­FóÝvÄ4ÈÓ³ÇÞ}4]ÿÛpÿ#Ž?}çá7ô¼þT×LÆÝ‚øàJÈ|óÀmîÃ`q8�IpÁä¥Ì±ô‚Çø�b"Ò�"KýÿM˜Õ66íi-5ô"KýÿM˜Õ66íi-5öKóþ^óöüÿ—¼ý+ÏÚï?Ø8yjªš˜Ñóì>á9¯E$„ê¨ù¨ÕS§Ø|IÔýÂ3ì÷órÿ@òs³Ï2³ÇŸóÜÏwËÁóÝ±0-ñ8Ô^ëíÌË±ÛB ,ÁÇó0¸sÁ,S 0ŠÏ°üÍƒÄ¡0¯ÃƒÌÓ]>ø3ÍðýA?2
ø=òÃ.ÏÇß@Ô€Ó<²;ü0ñ3<?>¾~ó?Í~ÿ®q0ì:ß-óÇ,B?3~Ø5?øsóýÇóLü|T7ö6ÏKm~àiÕj!Q¡àšéyŠ;êœæ g8êXú‰¶9£¯Éƒ«j»ï¢eo‚Ž.êa¢Ê-r‚#xŠŽ×ÿx(h÷ß‹Nú¼«:¿ûù£Ü0^Û<øú?8àt°â€ÞkÈ"‹ S‡/«Ø8ìøŠ=øã0¾¸ç/ëíoL¯¯Ì>8ðî0û¯2ë>³ûGÁ»ú€óïóàZÄì'êQõ‡§PÓ‰¤÷ª•EP xZÞýqGQ¸ê£¢x»‚‘ônà»ƒ—�÷Ž”ûYS£èHëRHˆü¸¢!î	y¿´ªKH�VîM$’¶-ºi­&¦^�QXY´D„Az*hÓ¦X)Õ•5“mÕ«ueMª«xÚä¢
‹Õíµ‰ºm‹ƒ¹9¿?åï?oÏù{ÏÒ¼ý®óýƒ‡–ª©©�ñ>ÃîšôRHAª��ŸÖ³ù’É§3;x<²©×,‡€Uþ§õélú4Ý¿ú³Óƒ%“Có|lþ¿](Í>X3ø“ÚS0Àˆ §3L‡Îw£Ó?“?;êÎ®¿¯«³Ü¬ðêÿ-Nål ïê@¯’:œ7ìÕW¡¤`­É =³ødpª³ú‡(÷+©d�îŠõx]í›°¹®¨jeª<jÃæ‚•V†¥ÕZ—1j—Uš\éÖ¤ Þ« V#0_ù¨©ª¹YÚÕ˜›ÎØGY†V:ÖY¡Ua&µÚ¼‹?¦¿ª£ª>¾*£·ôW8!RUáFÒ€�Ä€§l^ítÂQ¾„J¬ÿ\ªõ†z¥Jã˜ƒ0šoÉ¡ÏQ5ì,ógy®Ñaè(Iíô·ESnœ6%i�#�eöwŒ=zzá×—Îª�×`¶%ìú(ðNö}\q2÷ø“X5×(Í^ix1yéîN¿u¥‘à`CT£A÷®3°þ¼88*há•¸Â .Ì&Ú4Þ ô`”þü›$¦çqÀÐ×˜\VGFÎ��·†”Ú	x´}JžÙ™êF†¥^¶ªZ•A¹Q¾ªnØ}Vþ—!*²½á2Ñÿ$UT®„oª«ŒÉn8èÕÕU÷Î}0‚¢£ÁµŸ^ê¯%àëM4gÕž+®H“É)}ëQ{‰7Ï�Ÿ³¸å¾)ôƒŽ= ÆRÈº|
µd§Á”3L, 
ÈÝÓôè�ñxF~tÈNa3Üëñ@ECBo	L4ÐDÍÄëÐ\ø&êd¾,
³ß¸z,j¢ì´ 0O˜\ô×0ú©Â¾ª8>¨íØ®¯Œ¦ÿ†¸�—É§Ï¨HNó³Í<\|LÓÒ  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ,P<?xml version="1.0"?><Features nextValidID="27"><Feature recentID="26" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5062" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="4679" maxFusedRunOn="4679" detectionMode="exactProteinMatch"><Segment range="5063-5074" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="25" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5917-5933" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="M13 rev" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-227" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="187-203" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5889-5907" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="T3 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="248-266" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T3 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="113-134" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="149-154" color="#ffffff" type="standard"/><Segment range="155-172" color="#ffffff" type="standard"/><Segment name="-10" range="173-179" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6556-6660" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2095-2211" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5572-5693" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="748-873" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5733-5868" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3767-3902" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="521-701" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2538-2741" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="RSV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="294-520" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Rous sarcoma virus enhancer/promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5267-5500" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1366-1599" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2234-2537" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3587-3916" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6075-6530" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7692-8280" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4592-5180" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3926-4525" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="EGFP" directionality="1" translationMW="26942.08" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="2818-2820" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="2821-2823" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="2824-3537" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="AmpR" directionality="1" translationMW="31558.16" type="CDS" allowSegmentOverlaps="0" cleavageArrows="6729" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="6661-6729" color="#ccffcc" type="standard" translated="1"/><Segment range="6730-7521" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  =<Notes>
<UUID>9d09dad2-79cd-4df1-af32-0dbde0e69422</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="0:9:52">2018.9.13</Created>
<LastModified UTC="8:5:9">2024.7.3</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  pý7zXZ  æÖ´F !   t/å£áyT/] Ë‡ØÎf‘ƒÊý{3Ôé·Ú(1v%f M*	mj÷)p80çÌ*iÚ	´NY]Qó“�úF7î&ƒÕ!Ò÷ÀnãýJ„<Ú§K˜UhmŒçoð~Ðÿ"»Bâ�"{à±KU(-®£Ñ——êÄCODåîŠéOS^•�€�µs�g¦TB$9xãô�ŽxÓÊOò²þÿMè~$*7>¼š×Qÿ"Qz«&Ñ	N€ýˆþô€Æ�–@•®Tà¹ô1‘/¼ÔŠÕ~mæÕµ>Ò}"”ö"ÇúÑT¾lbZ‰F##ý»GñÃ»G=-ôV�®˜ü…Ü®’È��»ó.w¶“)»˜úŒ‘JùÂÐHù�kŠ6É–Eoiûµ’¢U¬	¾³üDðçÎö„)çq~™ÂÊ¬Óòsv|€ƒ ©$$«o“W¬'kÁ^BÔy£Ídè§óò+ßPÈ¥­Â’/¶÷]cç¸õ­ ãáH]²¸B^¹Ú‚2¥á8&"üFº˜Š#|IÕ‰Ú°“”7¦«ÓÆ›¦¾OAË‹fEdõ­ù.ÿÞŸ ;Ð•@óô÷Z)N÷Öa`Á~;Ô‰¡S¾
'�öGO»&¿Æñºþ$D	Ä[¸j3O= œeÉp˜Ðs…é±m%Æ—µè‹PS‰oýjÊÚì¢jý…cPân\Ü!Æ¿MÑ�)šjÖ¯ 	þl²Ôº:Q#ÒIÓÆíSån?QÄL^“U…ï4ó:±×>,`xÊ´ÛÁ+ö.�ÔØâƒ ç#‹ùì‡™ÑêÐ²îëõK&´­lUÆÞn7ÔØ$€RžzªhùÂnæ“ô‚ß`þÛÀ6@ÿô¿H{Ö9§Iðú0k'wZšy–|–ae¾¯–ks­…¸4ädV³6@Ó0Ìá\C•öc.{ºF>l4ÑJCDº<GŸ²@3b©
²jÚ7ìGÈTà�I«¸^�[Ü*Q˜B¯¬eØ28†%Cy¼:h�EÝY›¯ÊËäfŒ*°ÞÃLÙNÒI ÑM6*u[£~’U VVíFF@} Q¼„aµ&³ÑUÜIêú�Ù -ƒQƒ:--4¥m	.ÿCS•èÈÜHKå‹ŽâJ‰Ú>ÌðöPs
K0ÛB U<
[Çãc½cpždìcá£z¢|úÉ*àØÛ¶jnÇ,ñ¬b~÷È £–È}ëwïX¦ÊIÔ2pµF¯EKÎ°i*v¸ë^dè‹Ñ�0Dœ/‹¾!HfÿÃ˜ÎzÖæ¶‹/�DMÈìIÞRg3/¤D0@ã_[n?*iûƒnXúˆ¬aÕ ðÙÉïƒ%#Ëß�7½·µŒƒ±*×qÜd³;ÿ•øBffrœQ†fA¿\µ#ùZýˆwçÀ˜ÞqÅ“ò3.ò=|ã“"šh•1�–oÒÜ¥:„°)ðÿG}:Ãäù2ð8**ÃjåYOwÒÙ&a·à½(ü7` PRœÝnâAùM`ü¢©uà°œT$kõ­6>Þ1…¨&§@c>‚ä”âTA©s.ÎIpl§ói!s8FhêB£‹/)ó�–ë
Õ�¬°bú|©(.ÎñJ=¿‹—÷jah¯1¨Y£SŽG5ÅˆäŒ04IñlÈ~Êý~ˆ)`NAÛQ|‰èÚìpßc×Ê7Êx	±^éaH.Â‘A¹a¤>“.¤ @Ó€gfè+BR±P‰ˆÞÛT­N‡è'¬˜1V“õ?¯5ðø€�ÿ,­ôbîX¾(>†ˆä�´	s<`#�ž<ýLñØÍ:Ø¨Èx
†“{ÆûT‰Ý¶æ‡œh($÷@ÀJ‚_…”¬ZC�!4ãLÝŽ-h9È+¹…‡!=»,”ªZÇ<—§3ÃJ™:§íFaZWUó¸2€»6Sçìïõp)ÖsGtÛaÃŸÔ¸0”²úÉÊûÁœÂGçíÞèß`Z†&ŒØ¡Z1dGö‚‡;"™ƒDyÚEÑqd~j âÛ‘`”Ì{gv>ÍÕå‡<*/ö„,*Hz-åAmEMÒõyŽvÄUjEk›¬æg¾Õ¼òR‘nS‚LVÖúUJÊPìðXä8ñ×ãjÊs>H{�ð.ê¥™F9³5ÿËf) “xQ&8Ð;%d,­�õP˜ÜF}»óÜ$šòüV„ë�wçj f£÷h¡`5ÏX&‡¡ªz^½s}£”išƒ‰	IK’m¢£“°TUürh,v¬ÛƒK8îêncH1Ì|¥¥´2úI€oF™��)¿¿”x ™Éw¿Dè;tÑèßyÌ!+õ=­z(a·£ÈÀxÇ
&Q½ßXsÐx;Ä¿—È¤©€LXÝ)?¼uÅ_VÙîðù;"\ýÎ¯nwõ{m;ëY˜ö�RA’{a2Öß“ü´6kP ¦÷-²²Æ2o_sÙ+8Ÿ¬ „ŽX±=ÏïøâæÊàYs sŸƒ¦–3jõÒúf�¶Æaò Ðé©ûD‡Ÿ„Ù¼ç�	çd’£ØíŒ°Y9ƒT+÷YYG'&‘øØ84Ö Üê&jk–ÃÕß@:bxÑe€Lûu$ríôžöñ[{`-…�¨þ—�º)�‚ÆH®c›Ã9.?Ð—¥t`ëöÇ�©a8(Þ“‡UsNØK9oèmÓä»>’&Ÿ§„=Œ¶0C¬Qc~à÷Ì­ï[Ù½ �ì’�ç£.[÷ìOMøIFÚ¤"±á,Æ®•AœIn"×N3žÝ}*Ùå|Å'ý °}¼+Jz6ýqŠ›�Ò+5Oö[žØæ³�¹iÁ¯²)4ÆS÷àHŽé�S]¨~¢©ÏÚûœWÖ’j|LR¬TØ,$ˆq`V¦4lÐfø\àÿGD,óÙ­Ÿ^û	áÐä'“Q6½h›3IŠ©ªÑHhÀ»Æ¿†Î‚¦gÿò1ì=[›½§¤é÷=JÍÞ»ý /E/€áì§8èÐ©rxã½Œ!üS_¥¾á&·µYŒ,q)× �¢žÂ;öL@ØT·(U>y˜¥À¿6FÀKâ,ºÊ€¸$çH»fôTl¹È—4Yÿ­öýhjöï�`æ„nsøA×¤!Ë1˜‚ƒô½ÕÆå¸ªBågV`›ðwõÙwÛ"$ð´û·"ŸLoAºKKQ„Èx¹¯7ÆD`*ñg
›–êW¾œOf°Á¾0ÌmO²3UÍ¬…1žÐ¼$­¯fGM^ÛAÖÃV´ÈžvÏü–˜™‰0%£ºç¿ÐnM,¨&Œˆ$å�c©¯>~Ê~ž`Öÿ>æçº~™Cp2EÑÑ;(*S¯×Z\¸¸ãh¿fãoa_Æ�ìÂ›^›¬ÝÈrÈœ\÷$=£¢WÉ ×¸vÙ£ˆõBÃÙ\ÏtÙÂ!iTã˜-8ó¼
j )r ‚‘Iu°"/ŽqÛâðÇîeC(“y9K±Á�+1¸EbCè1ïtz5'n…Sur÷öÁê”!ì õø…w‰¶ìòíŽ·KÜ¸ôÝ4‰hG4ë�˜IÏ½(¤=3Éƒ)#µg3×·�ÍýÇöZöi›Û\Ä(S¹€¼ož‡O‚¦Ç«I2¿Ÿ[sÜÈž·“4h5ÛAÐs¡Ì+
ÄÃŒ´üQßÃ[M•o”ŒWç±Wú¢=,þ�p$ÊËòÚk©–Ì×GÑæ\ÿœŠˆDÏÖêa£î{BÉÊ¦nndŠ¯zatáÿ4¢"¯iÔ™>1ºk^îÇ‡«–ù)ÎüÛ8Ã*_×ø)€Dzq
IÿL¨iœoÑÛkõ´€ôŸ™�ñÅÄ|¢½ñxE¿À%çês¼þü2\K&�eÇ�"4ö9Ñvü¥‚®Ò]È–™×b ÇBÍ‘ÔŒ¡1p§vi7§ê”ì§îâk'ã2ˆ‡þË³‹¶À‡È0V¼Ò?ë•¤gIò÷M*¦ãb[¤O¿ÿ-A±9À©�"Â’wpä6c­.ml�¤ i#ìÍ „¤;�m_BÓ÷€Ñç!€Q®ó‹Ö€nX£Ìm¥gD>çšÞ×ûóÂ¬ßcçz¢*ô©Â'ÿæt¯«w‹·E›^»Uâ6‰P`ë=¢
¿M"TsƒÖŒž[¡ùÃ³Äa‡Ÿwe�gáýÇ°&Ê$Xº”Ø…ÊËBéŠ£�{Â¥òÈë@Üôwˆ 8µ€ìÕ¯~»ª°=¨t¶,îÌi†sÀ„‡ÕàC&ßüÐ÷ÿ¿˜d€7©™p6™ßFO™‡AKrj‰ º*Ö‚ÏJM?|»¾ìä“ Ò((ÏlŽª(j¾Ûé9'Ö¢Ý-ÕQ²k–�§I«;<f‘bt½Ð“¬á	þy÷N¨£K
Ì~R¢ä,tó�kÊ�ŸïÊ¸ÒÝøEGÂÚ ´Õþ°(ƒ—9 Ú~›Ä×´|Ö­n`ïuÃÕ*Ù$Y#·,"U·oò£d™0ÔéGé)MÈv÷\Éº-^ûçoÒí�áÊ¶µÞ+ø¸/šéŒ9¯‰óÍúŒ¸)Øøh¸Bª¡	1.Ÿ{o[•}–ëÛÓ.ø�Ý™õOï“L©kVElLü® Òù
$ÈàJ•õƒQévÀeŒÀ{¿ P9à‹„ù0ŠŸLÙèbÁ[À–Ai`G§öDð P3ÕÂ],ŸMì8fÙþ#Ÿ�Û¼÷y�ŸR|×n å?”^áZÀ±²ÒŒ¾M†ö%nN%\Œ�à£^¢ú{	©¦òq
È¹�‚¹�b”‡»Š¥åUÎÙq®7”Qf9õ„%«Z7šøjdØòV.bç-­ðMøPÒ­Òõ«Ç;º3 ­¢QÄ®OÊ2itË»f=ø4*#ÆR�ú†Ý6*h�ó+¸Dˆ%"E"û¶D	÷ž8¯EC²]|J³›c:ÀŒe"Q¾Pâ’þY�ú4— Ð nO;böè©€ õÉùÉ£Ù£Â«¶+{‰Ú¡ùÓ`‡å€¢¤½jCj•ªÅRÇXRR‚ûì{Ó/v’õÜWß×sN‰ã–ìkø'ŒÀúc|)ïLcÑK†éÉäê;(Ø±Ü<m(ÛŠ´Q_tš?=ª*ã_S…µNÝ15¥0Jz�¥•ªgÝó‹AAI¾@Ú‰ˆ>4Œ‚ªgÌåÆÛ
KÊ<,up ¼sÝu™ëd”¥mJâw%KX eðEå×Äs8>bf µ„’’s:¬\ù«¨ê³íHLÉIÝ÷X	¡³‹Lì¤¦¨q ¬®]3n•Ä3)N,àÃ!º'°«¸&æÀÎ¨$û°�6x«·]ñ*@Z«Ë;¾f^J5¸ý²s^	pÊUüVC›Ì1‚¹‹6I-Å¶e,48t)ªà=RÇaAR¹$¨Ý`Rñ™êV `¹ì5Ë~B[:ÎKOåúÅ°DfÚÕw±½7Qîå8&¹û1ß–:ÍQAz”|;¢ßM^r»=ÒË%°¿ Þò
RÔÂÊÊDLJ{ßZé+ à“ÎÏ²Ûêo®ä•îÌˆ´v²P#t6Z>¾{ýè~ê„Î¼„U+0˜g"°ÏçVÌO2›—åu¾HJ3½rBY¦Äºˆ‰JÝ5½Þ·“^>ž&\hô¢ºd¶9‘�‚¿£_?œÞcýEk˜ÝÅÎê¨	{½jL@{‚,ÎÖ±q9È:}M 2ŸW—B~ÜÍ@p«t�ðkÝÃÄyðeåW¢ugÂ`�å(Zù¿”Ø K„¹€PøÖùŽ+^*†&æT3�õˆ‘ŠvšØÿFSßzûªSm]†kLJ9lFS|h¶Å~‡5PF5¤{^“+æhØ°ô—h[¸~ZÀ«À…ß;¶ÔÛ�ºÇ˜Ži²z<ðzM¶›¡2ït_; $µ¶+‡shú/O¬á"ŒÓŒñÈÍÈÈ•¬&�·›«®F(äl]Îðíej*°DrÒ âIÀâûÖbH>sT·�àéÝæ¹ä¥¸^àÌùòñÈ7)þ±ê¢axózæ é-ÿÓÚ$jpY­`ÂÂ¶Oq™Ä°–àA.²…<ŒOªë™ÑšÒk2ËÌ™Ê;WnÞægtñåç¦cä*?N¨N²*Ór1g$¤‘‰t§¥_hJ8�½óÞZÏ<‰cd’+Ÿ4™iôä{hyÍ†é33õ@b?Ùj\ ½ˆoRƒpÊ>–UÿuÌd¾†q=ßÂNMš- @†>ª	YÓîö,¦»è     Òú¦Á¶ Ë$Õò >©†V±Ägû    YZ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ;Ã<?xml version="1.0"?><Features nextValidID="31"><Feature recentID="0" name="EGFP" directionality="1" translationMW="26942.08" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="3631-3633" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="3634-3636" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="3637-4350" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK*"/></Q></Feature><Feature recentID="1" name="NES" directionality="1" translationMW="1051.32" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4350" maxFusedRunOn="4350" detectionMode="exactProteinMatch"><Segment range="3595-3621" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;nuclear export signal from the HIV Rev protein (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/7543368&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/7543368&quot;&gt;Fischer et al., 1995&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="LPPLERLTL"/></Q></Feature><Feature recentID="2" name="attB1" directionality="1" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3562-3586" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;BP Clonaseâ„¢&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;mutant version of&amp;nbsp;&lt;i&gt;att&lt;/i&gt;B&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;recombination site for the GatewayÂ® BP reaction&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="Cycle 3 GFP" directionality="1" translationMW="26867.82" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" maxRunOn="4350" maxFusedRunOn="4350"><Segment range="2824-2826" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="2827-2829" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="2830-3540" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Cycle 3 GFP (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/9630892&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/9630892&quot;&gt;Crameri et al., 1996&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="M,A,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK"/></Q></Feature><Feature recentID="4" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2818-2827" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="5" name="AmpR" directionality="1" translationMW="31558.16" type="CDS" allowSegmentOverlaps="0" cleavageArrows="7540" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="7472-7540" color="#ccffcc" type="standard" translated="1"/><Segment range="7541-8332" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature><Feature recentID="6" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4737-5336" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"/></Q></Feature><Feature recentID="7" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5403-5991" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8503-9091" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6886-7341" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4398-4727" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2234-2537" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1366-1599" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="3' LTR (Î”U3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6078-6311" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="RSV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="294-520" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Rous sarcoma virus enhancer/promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2538-2741" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="521-701" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4578-4713" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6544-6679" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="HIV-1 Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="748-873" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6383-6504" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2095-2211" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7367-7471" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="149-154" color="#ffffff" type="standard"/><Segment range="155-172" color="#ffffff" type="standard"/><Segment name="-10" range="173-179" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="113-134" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="T3 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="248-266" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T3 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6700-6718" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="27" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="187-203" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="28" name="M13 rev" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="211-227" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="29" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6728-6744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="30" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5873" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="5490" maxFusedRunOn="5490" detectionMode="exactProteinMatch"><Segment range="5874-5885" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="IEGR"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ©<Notes>
<UUID>3fd7f8b6-fbc5-4db2-8f42-a606369961e5</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pLV3-CMV-2Ã—GFP-NES-SV40-Puro</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="0:9:52">2018.9.13</Created>
<LastModified UTC="10:13:10">2026.1.12</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y     K         
?????                                                                                                       ÿ d      DO  d              E              ÿÿÿÿYô? P                                                                                                                                                             Ã<StrandColors><TopStrand><ColorRange range="0..2816" colors="black"/><ColorRange range="2817..2833" colors="green"/><ColorRange range="2837..4347" colors="black"/><ColorRange range="4350..9265" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..2816" colors="black"/><ColorRange range="2817..2833" colors="green"/><ColorRange range="2837..4347" colors="black"/><ColorRange range="4350..9265" colors="black"/></BottomStrand></StrandColors>   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
